Device for testing solutions and body fluids

• US 3,901,657 A
• Filed: 04/29/1974
• Issued: 08/26/1975
• Est. Priority Date: 04/29/1974
• Status: Expired due to Term

• Current Assignee: Charles L. Lightfoot
• Original Assignee: Charles L. Lightfoot
• Inventors: Lightfoot, Charles L.
• Primary Examiner(s): Serwin, R. E.
• Application Number:
  US05/465,330
• Time in Patent Office: 484 Days
• Field of Search: 23/253TP,23B 116/114AM 252/408
• US Class Current: 422/400
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/9486 Analgesics, e.g. opiates, aspirine
• CPC Class Codes: Y10S 436/901 Drugs of abuse, e.g. narcotics, amphetamine
• CPC Class Codes: Y10T 436/141111 Diverse hetero atoms in same or different rings [e.g., alkaloids, opiates, etc.]

Abstract

A multi-layer device suitable for the colorimetric determination of the presence of a particular compound in a test solution is disclosed. The first layer contains an agent capable of concentrating the particular compound onto the surface of the layer, when the device is placed in a solution containing the particular compound. Subsequent reagent layers are incorporated as required to supply reagents, through diffusion, as needed to produce the end result of a color reaction on the first layer. Barrier layers may be incorporated between reagent layers. A new colorimetric method for the detection of morphine is also disclosed which may be adapted for use with the multi-layer device.

Claims

1. A REACTIVE TEST DEVICE CAPABLE OF CONVERTING MORPHINE TO ACHROMOGENIC COMPOUND COMPRISING A FIRST PORTION INCLUDING A MORPHINOPHILIC AGENT AND A SECOND PORTION INCLUDING A PERIODATE REAGENT FOR MORPHINE CAPABLE OF RACTING WITH MORPHINE TO PRODUCE A CHROMOGENIC COMPOUND WHICH PRODUCES A VISUALLY OBSERVABLE COLOR AT A PH OF ABOUT 11 TO 13.

2. The reactive test device of claim 1 further comprising a third portion including a color forming agent capable of reacting with the said chromogenic compound to produce a visually observable compound.

3. The reactive test device of claim 2 further comprising a non-reactive backing portion being at least substantially contiguous with the other portions.

4. The reactive test device of claim 3 wherein said backing portion includes a handle portion extending outward from said substantially contiguous portion.

5. The reactive test device of claim 2 wherein said morphinophilic agent is an acidic material, said periodate oxidizing agent is paraperiodic acid or its soluble salts and esters and pyridine and said color forming agent is a basic material.

6. A multi-portion test device suitable for the colorimetric detection of morphine in a solution by contact therewith comprising:

a first portion containing a morphinophilic agent whereby morphine in a contacted solution is concentrated in said first portion;

a second portion containing a reagent capable of converting morphine to a chromogenic compound, said reagent comprising pyridine and a compound selected from the group consisting of paraperiodic acid and its soluble salts and esters; and

a third portion containing a strong base said portions being arranged whereby when the test device is contacted with the said solution, the reagent, base and reaction products thereof may permeate through all the portions to form a visually observable color on said first portion.

7. The test device of claim 6 wherein at least one said portion is a strip of permeable, non-reactive material.

8. The test device of claim 7 wherein said test device further includes a rigid, impermeable material including a backing portion substantially contiguous with the said portions and a handle portion extending outwardly therefrom.

9. The test device of claim 6 wherein said morphinophilic agent is an acidic cationic exchange resin, said reagent capable of cOnverting morphine to a chromogenic compound is pyridine and paraperiodic acid and said strong base is sodium hydroxide or sodium methoxide.

10. A process for detecting morphine in a solution which comprises mixing said solution with a periodate reagent and pyridine to convert any morphine present to a chromogenic compound and contacting the resulting solution with a strong base to form a visually observable indication of the presence of morphine.

11. The process of claim 10 wherein said solution is a urine solution.

12. The process of claim 10 wherein said periodate reagent is paraperiodic acid or its salts and esters.

13. The process of claim 12 wherein said strong base is sodium hydroxide or sodium methoxide.