Method of and apparatus for chemically separating plasma or serum from formed elements of blood
First Claim
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1. A METHOD OF SEPARATING SERUM FROM THE FORMED ELEMENTS IN A BLOOD SAMPLE COMPRISING THE STEPS OF:
- COMBINING A LECTIN HEMAGGLUTININ, A POSITIVELY CHARGE POLYMER AND A BLOOD SAMPLE IN A CONTAINER, AGGLUTINATING THE FORMED ELEMENTS INTO CLUMPS WITH SAID LECTIN AND SAID POSITIVELY CHARGED POLYMER SUCH THAT EACH OF A PLURALITY OF FORMED ELEMENTS IN EACH CLUMP ARE AGGLUTINED WITH ANOTHER FORMED ELEMENT WITH A POLYMERIC
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Abstract
A process for separating plasma or serum from formed elements of the blood by adding a positively charged polymer and a lectin to a tube into which blood is drawn to effect precipitation of the formed elements of the plasma or serum.
31 Citations
15 Claims
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1. A METHOD OF SEPARATING SERUM FROM THE FORMED ELEMENTS IN A BLOOD SAMPLE COMPRISING THE STEPS OF:
- COMBINING A LECTIN HEMAGGLUTININ, A POSITIVELY CHARGE POLYMER AND A BLOOD SAMPLE IN A CONTAINER, AGGLUTINATING THE FORMED ELEMENTS INTO CLUMPS WITH SAID LECTIN AND SAID POSITIVELY CHARGED POLYMER SUCH THAT EACH OF A PLURALITY OF FORMED ELEMENTS IN EACH CLUMP ARE AGGLUTINED WITH ANOTHER FORMED ELEMENT WITH A POLYMERIC
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2. The method of claim 1 wherein said clumps of formed elements are combined to form a substantially integral mass after precipitating.
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3. The method of claim 2 wherein said clumps drag fibrin downwardly therewith while precipitating.
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4. A method of separating formed elements of the blood from serum comprising the steps of:
- combining a blood sample with a lectin hemagglutinin and a positively charged polymer in a container;
agglutinating the formed elements of the blood with said hemagglutinin and said positively charged polymer; and
precipitating the agglutinated formed elements of the blood so as to form a layer of serum above 99% of the formed elements of the blood in a given length of time, said layer having a total volume greater then the sum of the volume in a layer of serum above 99% of all elements in a blood sample of the same size combined with said lectin alone after the same given length of time plus the volume in a layer of serum above 99% of all elements of the blood in a blood sample of the same size combined with said positively charged polymer after the same given length of time.
- combining a blood sample with a lectin hemagglutinin and a positively charged polymer in a container;
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5. A method of separating formed elements of the blood from serum comprising the steps of:
- combining a blood sample with a lectin hemagglutinin and a positively charged polymer;
agglutinating the formed elements of the blood with said lectin and said positively charged polymer; and
precipitating the agglutinated formed elements of the blood at a rate in excess of the sum of the rates of precipitation achieved with said lectin alone in said blood sample and said positively charged polymer in said blood sample.
- combining a blood sample with a lectin hemagglutinin and a positively charged polymer;
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6. The method of separating plasma from formed elements of blood comprising the steps of:
- collecting a sample of blood and placing the sample in a blood collection container; and
reacting said blood sample with a lectin hemagglutinin and a positively charged polymer whereby the formed blood elements separate from the plasma.
- collecting a sample of blood and placing the sample in a blood collection container; and
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7. The method according to claim 6 wherein said positively charged polymer is Polybrene.
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8. The method according to claim 6 wherein said hemagglutinin is Bacto Phytohemagglutinin P.
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9. The method of claim 6 wherein the hemagglutinin and the positively charged polymer are simultaneously reacted with the blood.
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10. The method according to claim 6 wherein said blood collection container is hollow cylindrical in configuration and has a longitudinal axis and comprising the additional step of disposing said blood collection container with its longitudinal axis in a substantially horizontal plane to enhance precipitation of the formed blood elements from the plasma.
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11. The method according to claim 6 to produce serum, including the further step of adding a substance for inhibiting the formation of fibrin.
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12. The method according to claim 11 wherein said substance is thrombin.
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13. In combination, a blood collection container containing a hemagglutinin and a posItively charged polymer, said container having volumetric capacity sufficient to hold more than 30 cubic millimeters of blood.
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14. The combination according to claim 13 wherein the container also contains a substance for inhibiting the formation of fibrin.
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15. The combination of claim 13 wherein the said container is a vacuum type blood collection tube.
Specification