Processes for reproduction of cellular bodies

• US 3,906,929 A
• Filed: 12/12/1974
• Issued: 09/23/1975
• Est. Priority Date: 11/23/1973
• Status: Expired due to Term

• Current Assignee: Lynn Lawrence Augspurger
• Original Assignee: Lynn Lawrence Augspurger
• Inventors: Augspurger, Lynn Lawrence
• Primary Examiner(s): Medbery, Aldrich F.
• Application Number:
  US05/532,253
• Time in Patent Office: 285 Days
• Field of Search: 128/1R 424/85,100 195/1.8
• US Class Current: 600/34
• CPC Class Codes: A61D 19/04 for embryo transplantation
• CPC Class Codes: A61K 35/54 Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
• CPC Class Codes: Y10S 424/811 involving sex selection or contraception

Abstract

Disclosed are processes for reproduction of cellular bodies of herbivorous and omnivorous mammals. The techniques include obtaining ova, preparation of recipients and transplant techniques. Methods of sex selection of spermatozoa are shown as well as several techniques for clonal production of like embryos.

Claims

1. A process for embryo transplantation for increasing the number of progeny of female omnivorous and herbivorous hoofed mammal donors comprising, inducing superovulation in a desired donor by administration of gonadotrophin, fertilizing ova produced by said donor with a spermatazoa fraction of a desired sex, and transplanting selected ova or individual ovum derived from said donor to a selected recipient at the time that the recipient is synchronized with the development of the transplanted fertilized ovum or ova such that the transplant is synchronized at the blastocyst stage in the recipient at the time that the recipient would normally implant an ovum of its own had the recipient been fertilized.

2. A differentiated cellular body derived by the process according to claim 1.

3. In a processs for selecting the sex of offspring, the steps of utilizing spermatazoa as an antigen to form antibodies in a host, deriving a serum from said host containing said antibodies, mixing a titer of the serum with a spermatazoa fraction having a predominate fraction of a particular sex type, permitting inactivation of a portion of said predominate fraction, and to thus derive from the residue of the spermatazoa fraction an inseminate for use in fertilization of ova to yield offspring of which the selected sex is predominate.

4. A process according to the preceeding claim 3 wherein said residue is reused as an antigen in a subsequent host in a process for deriving offspring of a desired sex.

5. The inseminate product of the process according to claim 3.

6. A product according to claim 3 in combination with an embryo suitable for transplantation to a recipient.

7. A process for separation of spermatazoa to produce a fraction containing spermatazoa of a predetermined sex character comprising, washing sperm and transferring spermatazoa to a dillute fluid support solution, percollating the spermatazoa through and ion exchange material, concentrating a fraction of the percollated spermatozoa as the product and transferring the concentrated product to a buffered solution.

8. A process according to claim 7 wherein the ion exchange material is a cation exchange material.

9. A process according to claim 7 wherein the cation exchange material is a resin of the group consisting of carboxylic divinyl benzene copolymers, products of copolymerization of methacylic acid with divinyl benzene, and products of maleic anhydride with styrene and divinyl benzene.

10. A process according to claim 7 wherein the ion exchange material is a cation resin and the dillute fluid is percollated through the resin and the lower fraction utilized to provide the concentrated spermatazoa fraction.

11. A process according to claim 7 wherein the ion exchange material is a resin having the structure

12. A process according to claim 7 wherein the ion exchange material is a resin having the structure

13. A process according to claim 7 wherein the percollation is conducted under a vacuum of 15 to 30 cm. differential.

14. A process according to claim 7 wherein the percollation is conducted under a pressure.

15. A process for seperation of a desired spermatazoa fraction comprising, washing and dilluting the spermatazoa recovered from a donor, placing the dillute solution containing spermatazoa in a counterstreaming centrifuge and centrifuging for a period exceeding twenty minutes, during centrifugation exposing the spermatazoa to a second force, discontinuing centrifugation and removing the desired fraction from the dillutent.

16. A method according to claim 15 wherein the second force is a pressure differential from that pressure at which the spermotazoa was recovered.

17. A method according to claim 15 wherein the centrifugation is at a speed less than 1000 r.p.m.

18. A method according to claim 15 wherein the centrifugation is at a speed greater than 1000 r.p.m. and less than 1200 r.p.m.

19. A method according to claim 16 wherein the pressure differential is 15 to 30 c.m.

20. A method according to claim 15 wherein there is an ion exchange material within the centrifuge through which the spermatazoa percollates.

21. A process for Embryo transplantation according to claim 1 wherein the spermatazoa fraction is obtained by exposure of spermatozoa to serum containing antibodies to inactivate spermatazoa which will produce an undesired sex.

22. A process according to claim 21 wherein the fraction is obtained by the process which includes the steps of utilizing spermatazoa as an antigen to form antibodies in a host, deriving a serum from said host containing said antibodies, mixing a titer of the serum from said host with a spermatazoa fraction having a predominate fraction of a particular sex type, permitting inactivation of a portion of said predominate fraction, and thus deriving from the residue an inseminate fraction for use in said process.

23. A process according to claim 1 wherein a spermatazoa fraction which is used in the process is obtained by steps including, washing spermatazoa and transferring said spermatazoa to a dillute fluid support solution, percollating the spermatazoa in said dillute fluid through a collumn containing an ion exchange material, and concentrating the product fraction of spermatazoa passing through said collumn at a point in time and transferring the concentrated fraction to a buffered solution for subsequent use.

24. The product of the process according to claim 23.

25. A process according to claim 1 wherein a spermatazoa fraction which is used in the process is obtained by steps including, washing and dilluting the spermatazoa recovered from a donor, placing the dillute solution containing spermatazoa in a counterstreaming centrifuge and centrifuging for a period exceeding twenty minutes, during centrifugation exposing the spermatazoa to a second force, discontinuing centrifugation and removing the desired fraction from the dillutent.

26. The product of the process according to claim 25.