Device for determining the concentration of a substance in a fluid

• US 3,915,647 A
• Filed: 08/16/1974
• Issued: 10/28/1975
• Est. Priority Date: 08/16/1974
• Status: Expired due to Term

• Current Assignee: Richard F. Wright
• Original Assignee: Richard F. Wright
• Inventors: Wright, Richard F.
• Primary Examiner(s): Reese, Robert M.
• Application Number:
  US05/498,058
• Time in Patent Office: 438 Days
• Field of Search: 23/23B,253TP,23R,253R,259 116/114AM 195/13.5R,127
• US Class Current: 435/288.3
• CPC Class Codes: C12Q 1/48 involving transferase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: C12Q 1/58 involving urea or urease
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements

Abstract

A diagnostic test device which includes a cavity adapted to receive a fluid containing a substance to be tested, reagents adapted to contact said fluid and react with said substance to form a second substance capable of providing a visual determination of the presence and/or concentration of said first substance. In a preferred embodiment, chromatographic methods are employed to provide visual indication of the second substance.

Claims

1. A DEVICE FOR DETERMINING THE CONCENTRATION OF A SUBSTANCE IN A FLUID WHICH COMPRISES:

A FLUID RECEIVING CAVITY, A COLORIMETRIC INDICATOR SYSTEM, AND COMMUNICATING MEANS CONNECTING SAID CAVITY WITH SAID COLORIMETRIC INDICATOR SYSTEM, SAID COMMUNICATING MEANS INCLUDING A POROUS WICK.

2. The device of claim 1 wherein said colorimetric indicator system comprises precipitation chromatography.

3. A device as defined in claim 1 which includes, disposed in said fluid receiving cavity, reagents adapted to react with said substance to provide a visual indication of the concentration of said substance.

4. The device of claim 3 wherein said reagents comprise enzymes, coenzymes and colorimetric indicators.

5. The device of claim 4 wherein said wick contains said reagents.

6. The device of claim 5 wherein said reagents comprise hexokinase, glucose-6-phosphate dehydrogenase, adenosine triphosphate and nicotinamide adenine dinucleotide phosphate.

7. A device for determining the concentration of a substance in a fluid which comprises a cavity adapted to receive said fluid;

closure means for said cavity;

cavity volume indicating means associated with said cavity, reagents disposed in said cavity adapted to react with said substance to provide a visual indication of the concentration of said substance;

constricted passage means connecting said cavity and colorimetric indicator means where the visual indication of the concentration of the substance is adapted to be generated;

a porous wick retained in said passageway and a transparent cover sheet overlying said colorimetric indicator means.

8. The device of claim 7 wherein said wick comprises a microporous filter.

9. The device of claim 7 wherein said colorimetric indicator means comprises a precipitation chromatographic sheet.

10. The device of claim 7 wherein said transparent sheet overlying said colorimetric indicator means includes a scale indicating concentration standards for said substance.

11. The device of claim 7 which includes means for removing undesirable components from said fluid prior To contacting said reagents with said fluid.

12. The device of claim 7 wherein said device includes time delay means to provide contact between said reagents and said substance in a predetermined time sequence.

13. The device of claim 7 wherein said reagents comprise enzymes, coenzymes and colorimetric indicators.

14. The device of claim 13 wherein said reactants comprise hexokinase and glucose-6-phosphate dehydrogenase, adenosine triphosphate and nicotinamide adenine dinucleotide phosphate.