Diagnostic agent for the detection of peroxidatively active substances

• US 3,917,452 A
• Filed: 11/25/1974
• Issued: 11/04/1975
• Est. Priority Date: 12/20/1973
• Status: Expired due to Term

• Current Assignee: Hans-Georg Rey, Peter Rieckmann, Walter Rittersdorf, Werner Guthlein
• Original Assignee: Hans-Georg Rey, Peter Rieckmann, Walter Rittersdorf, Werner Guthlein
• Inventors: Rittersdorf, Walter, Guthlein, Werner, Rey, Hans-Georg, Rieckmann, Peter
• Primary Examiner(s): Reese, Robert M.
• Application Number:
  US05/526,661
• Time in Patent Office: 344 Days
• Field of Search: 23/23B,253TP 195/103.5 252/408 260/24D,24E
• US Class Current: 436/66
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 2326/00 Chromogens for determinations of oxidoreductase enzymes
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators

Abstract

Test strips for the detection of peroxidatively active substances in body fluids, e.g., for the detection of blood in urine, comprising a carrier containing A. A HYDROPEROXIDE; B. AT LEAST ONE CHROMOGEN; AND C. AS AN ACTIVATOR A COMPOUND OF THE FORMULA:

WHEREIN R1 is pyridyl or phenyl, optionally substituted by lower alkyl or alkoxy, and R2 is hydrogen or lower alkyl.

Claims

1. TEST STRIP COMPOSITION FOR THE DETECTION OF PEROXIDATIVELY-ACTIVE SUBSTANCES IN BODY FLUIDS, COMPRISING A CARRIER CONTAINING A HYDROPEROXIDE, AT LEAST ONE CHROMOGEN AND, AS AN ACTIVATOR, A COMPOUND OF THE FORMULA:

2. Test strip as claimed in claim 1 and wherein R1 in the formula of said activator compound is pyridyl.

3. Test strip as claimed in claim 1 wherein R1 in the formula of said activator compound is phenyl.

4. Test strip as claimed in claim 1 wherein R1 in the formula of said activator compound is pyridyl substituted by methyl or methoxy.

5. Test strip as claimed in claim 1 wherein R1 in the formula of said activator compound is phenyl substituted by methyl or methoxy.

6. Test strip as claimed in claim 1 wherein R2 in the formula of said activator compound is hydrogen.

7. Test strip as claimed in claim 1 wherein R2 in the formula of said activator compound is methyl.

8. Test strip as claimed in claim 1 wherein said activator compound is 2-(phenylvinyl)-4-methylpyridine.

9. Test strip as claimed in claim 1 wherein said activator compound is pyridyl-2-pyridyl-4-ethylene.

10. Test strip as claimed in claim 1 wherein said activator compound is 2-(4-methylphenylvinyl)-pyridine.

11. Test strip as claimed in claim 1 wherein said activator compound is 2-(4-methoxyphenylvinyl)-pyridine.

12. Test strip as claimed in claim 1 wherein said activator compound is di-(pyridyl-2)-ethylene.

13. Test strip as claimed in claim 1 wherein said activator compound is pyridyl-2-pyridyl-3-ethylene.

14. Test strip as claimed in claim 1 wherein said activator compound is Alpha -styryl-pyridine.

15. Test strip as claimed in claim 1 wherein said hydroperoxide is diisopropyl-benzene hydroperoxide or 2,5-dimethylhexane-2,5-dihydroperoxide.

16. Test strip as claimed in claim 1 wherein said indicator is of the benzidine series or of the heterocyclic azine series.

17. Test strip as claimed in claim 1 wherein said composition also contains a small amount of a complex forming agent.

18. Test strip as claimed in claim 1 wherein said composition also contains a thickening agent.

19. Test strip as claimed in claim 1 wherein said composition also contains a wetting agent.

20. Test strip as claimed in claim 1 comprising citrate buffer ethyLenediamine-tetraacetic acid, disodium salt dodecyl-benzene-sulfonic acid sodium salt 2,5-dimethylhexane-2,5-dihydroperoxide phosphoric acid trimorpholide o-tolidine o-stilbazole.

21. Test strip as claimed in claim 1 wherein the carrier is an absorbent material impregnated with the reagents.

22. Test strip as claimed in claim 21 wherein the carrier is a water-stable film with the reagents incorporated therein.

23. Method of detecting a peroxidatively active substance in a body fluid which method comprises contacting a sample of said body fluid with a test strip composition as claimed in claim 1 and observing a color change in said test strip composition as an indication of the absence or presence of the peroxidatively active substance.

24. Method as claimed in claim 23 wherein said peroxidatively active substance is blood.

25. Method as claimed in claim 23 wherein said body fluid is urine.