Method of carrying out enzyme-catalyzed reactions

• US 3,928,143 A
• Filed: 02/23/1972
• Issued: 12/23/1975
• Est. Priority Date: 02/23/1972
• Status: Expired due to Term

• Current Assignee: Robert W. Coughlin
• Original Assignee: Robert W. Coughlin
• Inventors: Coughlin, Robert W.
• Primary Examiner(s): Monacell, A. Louis
• Assistant Examiner(s): Wiseman, Thomas G.
• Application Number:
  US05/228,748
• Time in Patent Office: 1,399 Days
• Field of Search: 195/115,116,63,68
• US Class Current: 435/147
• CPC Class Codes: B01J 31/003 containing enzymes
• CPC Class Codes: C12N 11/00 Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: G01N 31/10 using catalysis

Abstract

A liquid stream containing the reactants is passed through a bed of enzyme catalyst, made up of enzymes bonded to small, dense particles of carrier or support materials, in a manner which causes the bed to expand or fluidize and a chemical reaction is thereby carried out in a process that is simultaneously free from limitations due to plugging and excessive pressure drop and which also has the advantage of the high mass transfer rates that can be realized between the liquid and small particles.

Claims

1. A METHOD OF CARRYING OUT ENZYMATIC REACTIONS USING ENZYMES ATTACHED TO INSOLUBLE, FINELY DIVIDED PARTICLES OF CARRIER MATERIAL WHICH COMPRISES CONTINUOUSLY FLOWING A LIQUID CONTAINING THE SUBSTRATE OR REACTANT UPWARDLY THROUGH A REACTION ZONE CONTAINING SAID ENZYME-BEARING PARTICLES AND MAINTAINING THE VERTICAL LIQUID VELOCITY WITHIN SAID ZONE SUFFICIENTLY HIGH TO CAUSE SUSPENSION AND AGITATION OF SAID PARTICLES BUT SUFFICIENTLY LOW TO ALLOW SAID PARTICLES TO REMAIN WITHIN SAID REACTION ZONE.

2. The method according to claim 1 wherein said particles also mix and circulate within said reaction zone.