Production of edible protein substances

• US 3,937,654 A
• Filed: 07/25/1975
• Issued: 02/10/1976
• Est. Priority Date: 05/14/1970
• Status: Expired due to Term

• Current Assignee: Ranks Hovis Mcdougall Limited
• Original Assignee: Ranks Hovis Mcdougall Limited
• Inventors: Solomons, Gerald L., Scammell, Gerald W.
• Primary Examiner(s): Monacell, A. Louis
• Assistant Examiner(s): Wiseman, Thomas G.
• Application Number:
  US05/599,026
• Time in Patent Office: 200 Days
• Field of Search: 195/31, 195/35, 195/81, 195/112, 195/79, 195/115, 195/76, 195/13, 426/48, 426/60
• US Class Current: 426/60
• CPC Class Codes: A23J 3/20 Proteins from microorganisms or unicellular algae
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: Y10S 435/804 Single cell protein
• CPC Class Codes: Y10S 435/929 Fusarium

Abstract

Process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of the gensus Fusarium or a variant or mutant thereof, in a culture medium containing essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance. Novel strains and variants of Fusarium graminearum are also disclosed.

Claims

1. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of the genus Fusarium or a variant or mutant thereof in a culture medium containing all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance possessing a high net protein utilization value of at least 70 based on the α

-amino nitrogen.

2. A process as claimed in claim 1 wherein the separated proliferated organism comprising the edible protein-containing substance is incorporated into a foodstuff for human or animal consumption.

3. A process as claimed in claim 1 wherein the substrate comprises starch, starch containing materials, or products of their hydrolysis, sucrose, sucrose containing materials, hydrolysed sucrose, or mixtures thereof.

4. A process as claimed in claim 1 wherein the substrate comprises hydrolysed potato, molasses, glucose, maltose, hydrolysed bean starch or whey.

5. A process as claimed in claim 1 wherein the temperature of incubation is between 25°

and 34°

C.

6. A process as claimed in claim 1 wherein the pH of the substrate medium during incubation is between 3.5 and 7.

7. A process as claimed in claim 1 wherein the process is carried out in continuous culture.

8. A process as claimed in claim 1 wherein the process is carried out in batch culture.

9. A process as claimed in claim 1 wherein at least one vitamin is present in the culture medium.

10. A process as claimed in claim 1 wherein the non-toxic strain of Fusarium is a strain of Fusarium graminearum.

11. A process as claimed in claim 9 wherein biotin is present.

13. A process as claimed in claim 1 wherein the non-toxic strain of the genus Fusarium is selected from the species consisting of Fusarium graminearum, Fusarium solani and Fusarium oxysporum or a variant or mutant thereof.

12. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions a non-toxic strain of the genus Fusarium or a variant or mutant thereof in a culture medium containing biotin and all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance possessing a high net protein utilization value of at least 70 based on the α

-amino nitrogen.

14. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of a variant I7, I-8, I-9, I-15 or I-16 of our parent strain of Fusarium graminearum Schwabe IMI 145425 deposited with the Commonwealth Mycological Institute and respectively assigned the numbers IMI 154209, IMI 154211, IMI 154212, IMI 154213 or IMI 154210 in a culture medium containing essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance.

15. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of Fusarium graminearum Schwabe deposited with the Commonwealth Mycological Institute and assigned the number IMI 145425 or a variant or mutant thereof in a culture medium containing all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance, possessing a high net protein utilization value of at least 70 based on the α

-amino nitrogen.

16. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of Fusarium solani or Fusarium oxysporum or a variant or mutant thereof in a culture medium containing all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance, possessing a high net protein utilization value of at least 70 based on the α

-amino nitrogen.

17. A method for the preparation of variants of Fusarium graminearum Schwabe IMI 145425 which comprises growing the parent strain IMI 145425 under continuous culture conditions with carbon limitation in a fermentation.

18. A method as claimed in claim 17 which comprises growing the parent strain IMI 145425 on a glucose based medium at 25°

to 30°

C under continuous culture conditions at a dilution rate of 0.10 to 0.15 hrs. ^-1 with carbon limitation in a fermentation for 1100 hours.

19. A method as claimed in claim 18 wherein the resulting proliferated variants are isolated by dilution plating.

20. A method for cultivating a strain of Fusarium graminearum Schwabe IMI 145425 or a mutant or variant thereof wherein the strain is present in a culture medium containing or being supplied with all nutrients or additives necessary for the sustenance and multiplication of the strain, the medium having a pH between 3.5 and 7 at a temperature of between 25°

and 34°

C and wherein the carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation.

21. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of the genus Fusarium or a variant or mutant thereof in a culture medium containing all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance possessing a high net protein utilization value of at least 65 based on the α

-amino nitrogen.

22. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of the genus Fusarium or a variant or mutant thereof in a culture medium containing biotin and all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance possessing a high net protein utilization value of at least 65 based on the α

-amino nitrogen.

23. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of Fusarium graminearum Schwabe deposited with the Commonwealth Mycological Institute and assigned the number IMI 145425 or a variant or mutant thereof in a culture medium containing all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance possessing a high net protein utilization value of at least 65 based on the α

-amino nitrogen.

24. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, non-toxic strain of Fusarium graminearum Schwabe deposited with the Commonwealth Mycological Institute and assigned the number IMI 145425 or a variant or mutant thereof in a culture medium containing all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferating, and separating the proliferated organism comprising the edible protein-containing substance.

25. A process for the production of an edible protein-containing substance which comprises incubating and proliferating, under aerobic conditions, a non-toxic strain of Fusarium solani or Fusarium oxysporum or a variant or mutant thereof in a culture medium containing all essential growth-promoting nutrient substances, of which carbon in the form of assimilable carbohydrate constitutes the limiting substrate in proliferation, and separating the proliferated organism comprising the edible protein-containing substance.