Radioimmune method of assaying quantitatively for a hapten
First Claim
1. A radioimmune method of assaying quantitatively for a hapten, the hapten having a ring of aromatic character, which ring is capable of having a --N=N-- group covalently bonded directly to an atom of said aromatic character ring, which method comprises contacting an aqueous sample containing the hapten under assay, a known quantity of a radioactively labeled marker which incorporates a hapten homologous to the hapten under assay, and an insolubilized antibody specific for the hapten under assay to competitively bind part of said labeled hapten and the hapten contained in said sample to the antibody;
- incubating the foregoing mixture until a two-phase system is produced comprising an insoluble or solid phase and an aqueous phase, and measuring the radioactivity of at least one of said two phases, the value of said radioactivity being a function of the concentration in said sample of the hapten under assay.
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Abstract
A radioimmune method of assaying quantitatively for a hapten comprising contacting an aqueous sample containing the hapten under assay, a known quantity of a radioactively labeled marker which incorporates a hapten homologous to the hapten under assay, and an insolubilized antibody specific for the hapten under assay to competitively bind part of said labeled hapten and the hapten contained in said sample to the antibody; incubating the foregoing mixture until a two-phase system is produced comprising an insoluble or solid phase and an aqueous phase, and measuring the radioactivity of at least one of said two phases, the value of said radioactivity being a function of the concentration in said sample of the hapten under assay.
178 Citations
1 Claim
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1. A radioimmune method of assaying quantitatively for a hapten, the hapten having a ring of aromatic character, which ring is capable of having a --N=N-- group covalently bonded directly to an atom of said aromatic character ring, which method comprises contacting an aqueous sample containing the hapten under assay, a known quantity of a radioactively labeled marker which incorporates a hapten homologous to the hapten under assay, and an insolubilized antibody specific for the hapten under assay to competitively bind part of said labeled hapten and the hapten contained in said sample to the antibody;
- incubating the foregoing mixture until a two-phase system is produced comprising an insoluble or solid phase and an aqueous phase, and measuring the radioactivity of at least one of said two phases, the value of said radioactivity being a function of the concentration in said sample of the hapten under assay.
Specification
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Current AssigneeBiological Developments Incorporated
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Original AssigneeBiological Developments Incorporated
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InventorsGross, Stanley Joseph
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Primary Examiner(s)Sebastian, Leland A.
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Application NumberUS05/160,559Time in Patent Office1,693 DaysField of Search424/1, 23/230 BUS Class Current436/518CPC Class CodesG01N 33/743 Steroid hormonesG01N 33/9406 NeurotransmittersY10S 436/804 Radioisotope, e.g. radioimm...Y10S 436/817 Steroids or hormonesY10S 436/823 Immunogenic carrier or carr...
