Continuous fermentation process

• US 3,940,492 A
• Filed: 06/25/1974
• Issued: 02/24/1976
• Est. Priority Date: 07/09/1973
• Status: Expired due to Term

• Current Assignee: ALFA Laval AB
• Original Assignee: ALFA Laval AB
• Inventors: Ehnstrom, Lars Karl Johan
• Primary Examiner(s): Monacell, A. Louis
• Assistant Examiner(s): Penland, R. B.
• Application Number:
  US05/483,007
• Time in Patent Office: 609 Days
• Field of Search: 426/28, 426/29, 426/30, 426/16, 195/17, 195/82, 195/115
• US Class Current: 426/16
• CPC Class Codes: C12C 11/07 Continuous fermentation
• CPC Class Codes: C12C 11/09 Fermentation with immobilised yeast
• CPC Class Codes: C12M 33/10 by centrifugation centrifuges in general B04B; Cyclones cyclones in general B04C
• CPC Class Codes: C12M 47/02 Separating microorganisms from the culture medium; Concentration of biomass separating microorganisms from their culture media C12N1/02
• CPC Class Codes: C12N 1/02 Separating microorganisms from their culture media
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y10S 435/813 Continuous fermentation

Abstract

Wort is continuously supplied to a circuit including an elongated closed channel and through which micro-organisms are fed. After fermentation has taken place in the circuit, the mixture of wort and micro-organisms is centrifuged to separate it into fermented wort, a living cell mass and impurities, these three components being separately discharged from the centrifuge. The separate discharges of fermented wort and living cell mass are continuous, and the latter discharge includes an excess of living cells formed in the circuit, an amount of cell mass corresponding to this excess being discharged from the circuit.

Claims

1. In carrying out continuous fermentation in a wort substrate, the method which comprises feeding a flow of micro-organisms in a circuit including an elongated closed channel, continuously supplying wort to said circuit, subjecting the mixture of wort and micro-organisms to a centrifugal separating operation after fermentation has taken place in the circuit, thereby separating said mixture into three components, namely, fermented wort, a living cell mass and impurities, separately discharging said impurities component from the centrifugal separating operation, and continuously and separately discharging from the separating operation said fermented wort component and said living cell mass including living cells formed in said circuit.

2. The method of claim 1, in which the centrifugal separating operation provides said discharged living cell mass with a dry substance content of at least 10 percent by weight.

3. The method of claim 1, which comprises also controlling the cell growth in said circuit by supplying oxygen in a controlled amount to the living cell mass being discharged from said separating operation, a controlled mixture of oxygen-containing gas and living cell mass being discharged from said operation by a paring step.

4. The method of claim 1, which comprises also controlling the time of contact between the cell mass and the wort in said circuit by controlling the flow through said circuit.

5. The method of claim 1, which comprises also maintaining different temperatures in different parts of said closed channel by effecting indirect heat exchange between the flow through said channel and a heat exchange medium.

6. The method of claim 1 for the production of alcohol, which also comprises discharging carbonic acid from at least one point of the closed channel.

7. The method of claim 1, which also comprises controlling the fermentation by supplying an additive to said closed channel.

8. The method of claim 1, in which said fermentation is carried out under superatmospheric pressure.