Electrical sensing circuitry for particle analyzing device

• US 3,944,917 A
• Filed: 08/13/1973
• Issued: 03/16/1976
• Est. Priority Date: 08/13/1973
• Status: Expired due to Term

• Current Assignee: Coulter Electronics, Inc. (Danaher Corporation)
• Original Assignee: Coulter Electronics, Inc. (Danaher Corporation)
• Inventors: Coulter, Wallace H., Liedholz, Gerhard A., Hogg, Walter R.
• Primary Examiner(s): Smith, Alfred E.
• Assistant Examiner(s): Hille, Rolf
• Application Number:
  US05/387,548
• Time in Patent Office: 946 Days
• Field of Search: 324/71 CP, 324/30 R, 324/64
• US Class Current: 324/71.1
• CPC Class Codes: G01N 15/132 Circuits
• CPC Class Codes: G01R 27/26 Measuring inductance or capacitance; Measuring quality factor, e.g. by using the resonance method; Measuring loss factor; Measuring dielectric constants ; Measuring impedance or related variables

Abstract

The electrical sensing circuitry includes a power supply and a signal-detecting circuit which are coupled through the resistance of an electrolyte in a liquid in an aperture of a particle-analyzing device between sensing electrodes positioned on either side of the aperture. The liquid containing an electrolyte and the passage of a liquidborne particle through the aperture causes a change in the resistance of the aperture thereby generating a signal which is detected by the signal-detecting circuit. The parameters of the circuit elements are chosen to provide circuit relationships which render the particle-generated signal independent of the diameter of the aperture. This is achieved by utilizing a power source having a low output impedance and a signal-detecting circuit which has a low input impedance for both D.C. and A.C., namely, at the signal frequencies of the signals sensed. The electrical circuitry also may include a conductivity monitoring circuit which may utilize one or more of the sensing electrodes and/or additional electrodes for monitoring changes in conductivity of the electrolyte and for relating these changes in conductivity to the particle-generated signals sensed by the signal-detecting circuit to alter those signals so as to render them independent of electrolyte resistivity.

Claims

1. Electrical sensing circuitry for a particleanalyzing device wherein liquid containing particles is caused to flow through a sensing aperture on either side of which is located a sensing electrode, said circuitry being independent of slow changes in aperture diameter and including constant voltage means coupled to said electrodes for establishing an electric excitation current through said aperture and means coupled to said electrodes for detecting signals generated by particles passing through said aperture, said means for establishing an electric excitation current through said aperture having a low output impedance at D.C.

and said means coupled to said sensing electrodes for detecting signals generated by particles passing through said aperture having a low input impedance at the frequencies of the particle-generated signals, said circuitry including circuit means for coupling said signal-detecting means, said electrical excitation current establishing means, and the voltage potential across said aperture in such a manner that the D.C. component of the excitation current flowing through said aperture is made to vary inversely proportional to slow changes of the steady state resistance of the resistance across said sensing electrodes through said aperture with voltage across said sensing electrodes remaining constant.

2. The circuitry according to claim 1 wherein said signal-detecting means comprises a current-sensitive amplifier.

3. The circuitry according to claim 1 wherein said means for establishing the electrical excitation current includes a direct current power supply.

4. The circuitry according to claim 1 wherein said means for establishing said electrical excitation current includes an alternating current power supply.

5. The circuitry according to claim 1 including means coupled to said signal-detecting means for monitoring the conductivity of the liquid containing particles and for altering the output signals from said signal-detecting means relative to changes in liquid conductivity thereby to render said output signals independent of liquid conductivity.

6. The circuitry according to claim 1 wherein said means for establishing electrical excitation current to said aperture includes a power electrode adjacent to each one of said sensing electrodes, a power supply connected in series with said power electrodes, circuit means coupled to said sensing electrodes and including a reference voltage source and a comparator which compares the voltage across said sensing electrodes through said aperture with the voltage from said reference source and feedback circuit means connected between the output of said comparator and said power supply for altering the output of said power supply to adjust for changes in the voltage across said sensing electrodes so as to maintain the voltage across said sensing electrodes equal to the voltage from on said reference voltage source.

7. The circuitry according to claim 6 wherein said signal-detecting means includes a low input impedance amplifier connected between one of said power electrodes and said power supply.

8. The circuitry according to claim 6 wherein said signal-detecting means includes an amplifier connected across said sensing electrodes, said amplifier having a low input impedance at the frequencies of the particlegenerated signals, and a high impedance at D.C.

9. The circuitry according to claim 6 wherein said feedback loop comprises an R - C filter, a chopper-inverter, an A.C. power amplifier and a transformer connected in series between said comparator and said power supply.

10. The circuitry according to claim 1 wherein said means for establishing an electric excitation current includes an A.C. power supply and an inductance in series with said sensing electrodes and said signal-detecting means includes a capacitance and a low impedance amplifier in series with said sensing electrodes.

11. The circuitry according to claim 5 wherein said conductivity monitoring means includes a conductivity cell comprising two electrodes situated in the liquid in which one of said sensing electrodes is situated.

12. The circuitry according to claim 11 wherein the output of said signal-detecting means is connected to a modulator which modulates the output signal with a high frequency carrier, a current output stage connected to said modulator, and a demodulator, said current output stage having an output connected to one of said conductivity cell electrodes and to said demodulator, the other of said conductivity cell electrodes being connected to a common conductor for said circuitry.

13. The circuitry according to claim 5 wherein said conductivity monitoring means includes conductivity cell which is physically isolated from the sensing zone including said sensing aperture.

14. The circuitry according to claim 11 wherein said conductivity cell is long and narrow with each of said conductivity cell electrodes being located at one end of said cell.

15. The circuitry according to claim 14 wherein said conductivity cell includes a serpentine tubing communicating with the liquid in which said one sensing electrode is situated and said conductivity cell electrodes being located at either end of said tubing and communicating with the electrolyte within said tubing.

16. The circuitry according to claim 15 including a third electrode which constitutes one of said conductivity cell electrodes and which is located in said tubing intermediate the ends thereof and wherein said end electrodes together constitute the other of said conductivity cell electrodes and are connected together and to the common conductor for said circuitry.

17. The circuitry according to claim 1 wherein said electric current excitation establishing means includes an A.C. source, a first inductance and a first capacitance connected in series and said signal-dectecting means includes a second capacitance and a second inductance connected in series with said sensing electrodes and said electric current excitation establishing means, and said signal detecting means further includes a transformer the primary winding of which constitutes said second inductance, a low impedance amplifier connected to the secondary winding of said transformer, a frequency changer having a high output impedance connected to the output of said amplifier, and a conductivity cell formed between one of said sensing electrodes and a third electrode, the output of said frequency changer being connected to said third electrode and to a demodulator such that the output signal from said demodulator is independent of the aperture diameter and independent of the conductivity of the liquid containing the particles.

18. The circuitry according to claim 11 wherein said electric current citation establishing means includes a low impedance power source and said signal-detecting means includes a low impedance amplifier, said power supply and said amplifier being connected in series between one sensing electrode and a common conductor for said circuitry, the other sensing electrode being connected to said common conductor and forming one electrode of said conductivity cell, the other conductivity cell electrode being connected to the input of a tuned amplifier, an A.C. source and a capacitor connected in series between said common conductor and said input of said tuned amplifier, a multiplier having two inputs and an output, a rectifier filter connected between said tuned amplifier output and one of said multiplier inputs and the output of said low impedance amplifier being connected through a capacitance to the other multiplier input which input is also connected to a load resistance connected to said common conductor.

19. Electrical sensing circuitry for a particle-analyzing device wherein liquid containing particles is caused to flow through a sensing aperture on either side of which is located a sensing electrode, said circuitry including means coupled to said electrodes for establishing an electric excitation current through said aperture, means coupled to said electrodes for detecting signals generated by particles passing through said aperture and electrical monitoring means coupled to said signal-detecting means for monitoring the conductivity of the liquid containing particles and for electrically altering the output signals from said signaldetecting means relative to changes in the liquid conductivity, said circuitry including a modulator, the output of said signal detecting means being connected to said modulator which superimposes the output signals on a carrier frequency, said modulator having a current output stage coupled to a demodulator, and said conductivity monitoring means including a pair of conductivity sensing electrodes forming a conductivity cell in the liquid containing particles, said conductivity cell being connected to the input of said demodulator whereby the particle-generated signals on the carrier frequency at the input to the demodulator are simultaneously applied to a load resistance dependent upon conductivity of the liquid so that the output signals from said demodulator are independent of liquid conductivity.

20. Electrical sensing circuitry for a particle-analyzing device wherein liquid containing particles is caused to flow through a sensing aperture on either side of which is located a sensing electrode, said circuitry including means coupled to said electrodes for establishing an electric excitation current through said aperture, means coupled to said electrodes for detecting signals generated by particles passing through said aperture and electrical monitoring means coupled to said signal-detecting means for monitoring the conductivity of the liquid containing particles and for electrically altering the output signals from said signaldetecting means relative to changes in the liquid conductivity thereby to render said output signals independent of liquid conductivity, said circuitry further including a modulator, the output of said signaldetecting means being connected to said modulator which superimposes said output signals on a carrier frequency, said modulator having a current output stage coupled to the input of a demodulator through the primary of a transformer and an inductance, a capacitor being connected between said demodulator input and the common conductor for said circuitry, and said conductivity monitoring means including a conductivity cell defined by two electrodes situated in the liquid in which one of said sensing electrodes is situated, said conductivity cell being connected across the secondary of said transformer.

21. Electrical sensing circuitry for a particle-analyzing device wherein liquid containing particles is caused to flow through a sensing aperture on either side of which is located a sensing electrode, said circuitry including means coupled to said electrodes for establishing an electric excitation current through said aperture, means coupled to said electrodes for detecting signals generated by particles passing through said aperture and electrical monitoring means coupled to said signal-detecting means for monitoring the conductivity of the liquid containing particles and for electrically altering the output signals from said signaldetecting means relative to changes in the liquid conductivity thereby to render said output signals independent of liquid conductivity, and wherein said signal-detecting means includes an amplifier, and a first capacitance and a first resistance connected across said electrodes, the junction between said first capacitance and said first resistance being connected to the input of said amplifier, and said conductivity monitoring means including said sensing electrodes, a second resistance and a second capacitance connected across said sensing electrodes, and a divider for coupling said conductivity monitoring means to said signal-detecting means, the output of said amplifier being connected to the numerator input of said divider and the junction between said second resistance and said second capacitance being connected to the denominator input of said divider.

22. Electrical sensing circuitry for a particleanalyzing device wherein liquid containing particles is caused to flow through a sensing aperture on either side of which is located a sensing electrode, said circuitry including means coupled to said electrodes for establishing an electric excitation current through said aperture, means coupled to said electrodes for detecting signals generated by particles passing through said aperture and electrical monitoring means coupled to said signal-detecting means for monitoring the conductivity of the liquid containing particles and for electrically altering the output signals from said signaldetecting means relative to changes in the liquid conductivity thereby to render said output signals independent of liquid conductivity, and wherein said signal-detecting circuit includes a capacitor and a resistance connected across said electrodes and an amplifier connected to the junction between said capacitor and resistance, and said conductivity monitoring means includes said sensing electrodes, an oscillator and a capacitor connected in series across said electrodes, a capacitance and a inductance series tuned to the frequency of said oscillator and connected between the output of said amplifier and the common conductor for said circuitry, and a divider for coupling said conductivity monitoring means to said signal-detecting means, the junction between said capacitance and inductance being connected through a phase-sensitive filter to the denominator input of said divider and the output of said amplifier being connected to the numerator input of said divider.

23. Electrical sensing circuitry for a particle-analyzing device wherein liquid containing particles is caused to flow through a sensing aperture on either side of which is located a sensing electrode, said circuitry including means coupled to said electrodes for establishing an electric excitation current through said aperture, means coupled to said electrodes for detecting signals generated by particles passing through said aperture and electrical monitoring means coupled to said signal-detecting means for monitoring the conductivity of the liquid containing particles and for electrically altering the output signals from said signal-detecting means relative to changes in the liquid conductivity thereby to render said output signals independent of liquid conductivity, said means for monitoring conductivity including a conductivity cell defined by a pair of conductivity sensing electrodes in the liquid, said conductivity cell forming a resistance which is connected as a load resistance to the output of said signal-detecting means, and wherein said conductivity cell is physically isolated from the sensing zone including said sensing aperture.

24. The circuitry according to claim 23 wherein said conductivity cell is long and narrow with each of said conductivity cell electrodes being located at one end of said cell.

25. The circuitry according to claim 24 wherein said conductivity cell includes a serpentine tubing communicating with the liquid in which said one sensing electrode is situated and said conductivity cell electrodes being located at either end of the tubing and communicating with the electrolyte within said tubing.

26. The circuitry according to claim 25 including a third electrode which constitutes one of said conductivity cell electrodes and which is located in said tubing intermediate the ends thereof and wherein said end electrodes together constitutes the other of said conductivity cell electrodes and are connected together and to the common conductor for said circuitry.

27. In a particle study device wherein a liquid electrolyte containing particles is caused to traverse an electrical sensing zone of small dimensions and wherein said device has a conductivity cell including two electrodes in the electrolyte for establishing a variable resistance which is the function of the conductivity of the electrolyte and which is connected to electrical sensing circuit including the sensing zone to provide compensation for changes in electrolyte conductivity, the improvement comprising said conductivity cell including a long and narrow column of electrolyte between said electrodes with each of said electrodes being in contact with the electrolyte at one end of said column.

28. The conductivity cell according to claim 27 including a length of serpentine tubing and wherein said long and narrow column of electrolyte is defined by the electrolyte within said serpentine tubing, said electrodes being located at each end of said tubing and being in contact with the electrolyte in said tubing.

29. The conductivity cell according to claim 28 including a third electrode which constitutes one of said conductivity cell electrodes and which is located in said tubing intermediate the ends thereof and wherein said end electrodes together constitute the other of said conductivity cell electrodes and are connected together and to the common conductor for said circuitry.

30. The particle study device according to claim 27 wherein said conductivity cell is physically isolated from the sensing zone.