Method of preparing tissue for microscopic examination
First Claim
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1. In a method of preparing a specimen of tissue for microscopic examination including the steps of:
- immersing a specimen in a bath of dehydrating agent;
immersing the dehydrated specimen in a bath of clearing agent; and
immersing the cleared specimen in a bath of paraffin, the improvement comprising;
the step of applying ultrasonic energy to said specimen during each of said steps, whereby the time required to prepare said specimen is reduced.
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Abstract
In a process for preparing tissue for microscopic examination which process includes the steps of fixing a specimen of tissue, dehydrating the fixed specimen, clearing the dehydrated specimen, and impregnating the cleared specimen with paraffin, ultrasonic energy is applied to the specimen in each of the processing steps to reduce the total preparation time. Also, the time required to stain a specimen of tissue is reduced when ultrasonic energy is applied to the specimen while immersed in the stain.
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6 Claims
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1. In a method of preparing a specimen of tissue for microscopic examination including the steps of:
- immersing a specimen in a bath of dehydrating agent;
immersing the dehydrated specimen in a bath of clearing agent; and
immersing the cleared specimen in a bath of paraffin, the improvement comprising;
the step of applying ultrasonic energy to said specimen during each of said steps, whereby the time required to prepare said specimen is reduced. - View Dependent Claims (2, 3, 4, 5, 6)
- immersing a specimen in a bath of dehydrating agent;
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