Multi-channel optical time-sharing apparatus having a rotating filter wheel with position-encoding means

• US 3,963,351 A
• Filed: 04/14/1975
• Issued: 06/15/1976
• Est. Priority Date: 04/14/1975
• Status: Expired due to Term

• Current Assignee: Britton Chance, John R. Sorge, Norman C. Graham Jr, Victor Legallais
• Original Assignee: Britton Chance, John R. Sorge, Norman C. Graham Jr, Victor Legallais
• Inventors: Chance, Britton, Sorge, John R., Graham, Norman C. Jr., Legallais, Victor
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US05/567,946
• Time in Patent Office: 428 Days
• Field of Search: 356/73, 356/85, 356/188, 250/226, 250/227, 250/231 SE, 250/233, 250/458, 250/461, 250/461 B
• US Class Current: 356/317
• CPC Class Codes: G01J 2001/444 Compensating; Calibrating, e.g. dark current, temperature drift, noise reduction or baseline correction; Adjusting
• CPC Class Codes: G01J 2003/123 Indexed discrete filters
• CPC Class Codes: G01N 2021/3162 with offset adjustment between filters
• CPC Class Codes: G01N 2021/3174 Filter wheel
• CPC Class Codes: G01N 2021/4742 comprising optical fibres
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 2201/0621 Supply
• CPC Class Codes: G01N 2201/0806 Light rod

Abstract

A multi-wavelength time-sharing apparatus usable in various different configurations of optical measuring equipment to provide a plurality of time-shared optical channels and including appropriate circuitry. The apparatus employs a turbine-driven filter wheel having a shaft position-encoding arrangement consisting of a hollow shaft portion which is internally illuminated. The hollow shaft portion has two sets of spaced apertures cyclically communicating with respective angularly spaced pairs of externally fixedly mounted phototransistors which are sequentially illuminated as the shaft portion rotates and which produce timing pulses which drive Schmitt triggers, which, in a typical embodiment, in turn produce pulses compatible with a logic circuit employed to decode the phototransistor signals into triggers for driving a 4-channel switched gain equalizer, a dark current clamp, and detector gates. Appropriate selection of the signals enables the instrument to function as a fluorometer or a spectrophotometer.

Claims

1. In a multi-wavelength optical instrument of the type including a plurality of optical channels provided with respective gain equalizer means and signal detection means, a rotating filter member having a plurality of wavelength filters spaced around its axis to define said optical channels and provided with a supporting shaft having a hollow portion, means to illuminate said hollow shaft portion internally, respective signal-generating photosensitive means associated with the gain equalizer means and the signal detection means mounted externally adjacent said hollow shaft portion, said hollow shaft portion having means formed to illuminate said respective photosensitive means so as to generate respective cyclic sets of long and short pulses, and means to decode said pulses to derive respective cyclic gating pulses for said gain equalizer means and signal detection means.

2. The multi-wave optical instrument of claim 1, and wherein said instrument includes dark current clamp means and said decoding means includes means to derive cyclic gating pulses for said dark current clamp means from said first-named sets of pulses.

3. The multi-wave optical instrument of claim 1, and wherein the means to illuminate said hollow shaft portion internally comprises a body of transparent light-diffusing material substantially filling said hollow shaft portion and having an exposed surface, and a light source mounted adjacent said exposed surface.

4. In a multi-wave optical instrument of the type including a plurality of optical channels provided with respective gain equalizer means and respective measure signal detection means, a rotating filter member having a plurality of wavelength filters spaced around its axis to define said optical channels and provided with a supporting shaft having a hollow portion, means to illuminate said hollow shaft portion internally, first signal-generating photosensitive means mounted adjacent said hollow shaft portion, said hollow shaft portion having means formed to illuminate said first photosensitive means so as to generate cyclically relatively long pulses, second signal-generating photosensitive means mounted adjacent said hollow shaft portion, said hollow shaft portion having means formed to illuminate said second photosensitive means so as to generate cyclic sets of short pulses, and means to decode said long pulses and short pulses respectively to derive cyclic gating pulses for said gain equalizer means and cyclic gating pulses for said measure signal detection means.

5. The multi-wave optical instrument of claim 4, and wherein the first photosensitive means comprises a pair of angularly spaced phototransistors and the hollow shaft portion has a relatively long peripherally extending aperture arranged sequentially to illuminate said spaced phototransistors.

6. The multi-wave optical instrument of claim 5, and wherein said second signal-generating photosensitive means comprises a phototransistor and said hollow shaft portion is formed with spaced apertures corresponding in number to the number of filters and arranged sequentially to illuminate said last-named phototransistor.

7. The multi-wave optical instrument of claim 4, and wherein said instrument includes dark current clamp means and said decoding means includes means to derive cyclic gating pulses for said dark current clamp means from said short pulses.

8. The multi-wave optical instrument of claim 7, and wherein said second photosensitive means comprises a pair of angularly spaced phototransistors, said hollow shaft portion being formed with spaced apertures corresponding in number to the number of effective filters and arranged sequentially to illuminate said spaced phototransistors to derive cyclic pairs of short pulses, said decoding means including means to derive respective time-spaced cyclic gating pulses for the measure signal detection means as well as for the dark current clamp means from said cyclic sets of short pulses.

9. The multi-wave optical instrument of claim 4, and wherein said instrument includes dark current clamp means and wherein the first photosensitive means comprises a first pair of angularly spaced phototransistors and the hollow shaft portion has a relatively long peripherally extending aperture arranged sequentially to illuminate said spaced phototransistors, whereby to generate said relatively long pulses, and wherein said second photosensitive means comprises a second pair of angularly spaced phototransistors, said hollow shaft portion being formed with spaced apertures corresponding in number to the number of effective filters and arranged sequentially to illuminate said second pair of phototransistors, whereby to generate said cyclic sets of short pulses, said decoding means including means to derive respective time-spaced gating pulses for the gain equalizer means from the long pulses and means to derive respective time-spaced cyclic gating pulses for the measure signal detection means and for the dark current clamp means from the cyclic sets of short pulses.

10. The multi-wave optical instrument of claim 9, and wherein said relatively long peripherally extending aperture comprises a slot of approximately 180°

in angular extent formed in the wall of said hollow shaft portion.

11. The multi-wave optical instrument of claim 9, and wherein said first pair of phototransistors are spaced apart by approximately 90°

.

12. The multi-wave optical instrument of claim 9, and wherein said second pair of phototransistors are spaced apart by approximately 45°

.

13. The multi-wave optical instrument of claim 9, and wherein the instrument includes a substantially Y-shaped light pipe having light beam-transmitting legs with ends located to register simultaneously with a pair of spaced filters carried by said rotating filter member.

14. The multi-wave optical instrument of claim 4, and wherein the instrument is provided with respective supporting ring members surrounding said hollow shaft portion, means for adjustably securing said ring members in fixed positions, and means for mounting said first and second photosensitive means in said ring members in positions to be cyclically illuminated by said hollow shaft portion.

15. The multi-wave optical instrument of claim 4, and wherein the means to illuminate said hollow shaft portion internally comprises a body of transparent light-diffusing material substantially filling said hollow shaft portion and having an exposed end, and a light source mounted adjacent said exposed end, the wall of the hollow shaft portion having an approximately 180°

peripheral slot adjacent the first photosensitive means, and the wall of said hollow shaft portion having a plurality of evenly angularly spaced apertures corresponding in number to that of the filters adjacent the second photosensitive means.

16. The multi-wave optical instrument of claim 15, and wherein the first photosensitive means comprises a first pair of phototransistors spaced apart by approximately 90°

and the second photosensitive means comprises a second pair of phototransistors spaced apart by approximately 45°

.

17. In a spectrofluorometer, a rotating filter member having a plurality of wavelength filters spaced around its axis, and respective excitation and fluorescent emission detection light path-defining means arranged to simultaneously transmit excitation light through one wavelength filter and fluorescence emission light through another wavelength filter, said light pathdefining means being at radial distances from the axis of rotation of said rotating filter member sufficiently different to prevent phosphorescence induced in said one filter from appearing in the fluorescence light path-defining means.