Diagnostic composition for the determination of glucose

• US 3,964,870 A
• Filed: 03/04/1975
• Issued: 06/22/1976
• Est. Priority Date: 03/29/1974
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Lange, Hans, Tiedemann, Hugo, Rieckmann, Peter, Rey, Hans-Georg, Sojka, Bernward
• Primary Examiner(s): Reese, Robert M.
• Application Number:
  US05/555,327
• Time in Patent Office: 476 Days
• Field of Search: 23/253 TP, 23/230, 23/DIG. 2, 195/103.5 C
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/54 involving glucose or galactose

Abstract

Glucose contained in urine is determined by contacting a sample with a diagnostic composition comprising an absorbent carrier impregnated with glucose oxidase, peroxidase, an indicator compound such as o-tolidine, and as a buffer, 2-(N-morpholino)-ethane-sulfonic acid.

Claims

1. Diagnostic agent for the detection of glucose in urine, comprising an absorbent carrier impregnated with glucose oxidase, peroxidase, an indicator compound and, as a buffer, 2-(N-morpholino)-ethane-sulfonic acid.

2. Diagnostic agent as claimed in claim 1, wherein the absorbent carrier is filter paper.

3. Diagnostic agent as claimed in claim 1, wherein said indicator compound is o-tolidine.

4. Diagnostic agent as claimed in claim 1 also containing a solubilizing agent or a wetting agent.

5. Diagnostic agent as claimed in claim 1, wherein a yellow colored material is additionally present.

6. Diagnostic agent as claimed in claim 1, wherein the impregnated absorbent carrier is sealed between two synthetic resin films.

7. Diagnostic agent as claimed in claim 1, wherein the impregnated absorbent carrier is sealed or adhered to a synthetic resin film.

8. Diagnostic agent as claimed in claim 1, wherein the impregnated absorbent carrier is sealed between a synthetic resin film and a fine-mesh material.

9. Diagnostic agent as claimed in claim 1, wherein the pH value of the buffer is between 5.8 and 6.2.

10. Diagnostic agent as claimed in claim 9, wherein the pH value of the buffer is about 6.0.