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Enzymatic determination of glucose

  • US 3,964,974 A
  • Filed: 09/10/1973
  • Issued: 06/22/1976
  • Est. Priority Date: 09/28/1972
  • Status: Expired due to Term
First Claim
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1. An analytical agent for the quantitative enzymatic determination of glucose, consisting essentially of:

  • a. glucose dehydrogenase having a dry weight activity of at least 2 U/mg., the NAOH2 oxidase activity of which is less than 0.1% of said glucose dehydrogenase activity;

    b. a pyridine coenzyme selected from the group consisting of NAD, NADP, thio-NAD, thio-NADP, nicotinamide purine dinucleotide, nicotinamide-(6-methylpurine)-dinucleotide and nicotinamide-(2-chloro-6-methylpurine)-dinucleotide in an amount sufficient to provide about a 2.0-5.0 millimole concentration in an analytical test solution;

    c. means for maintaining an aqueous test solution of said analytical agent at a pH of about 6.5-8.5; and

    d. mutarotase in an amount sufficient to increase the spontaneous mutarotation of α

    -glucose to β

    -glucose in said test solution by more than 50%.

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