Method and apparatus for measuring one or more constituents of a blood sample

• US 3,972,614 A
• Filed: 07/10/1974
• Issued: 08/03/1976
• Est. Priority Date: 07/10/1974
• Status: Expired due to Term

• Current Assignee: Radiometer A/S (Danaher Corporation)
• Original Assignee: Radiometer A/S (Danaher Corporation)
• Inventors: Johansen, Ebbe, Lundsgaard, Finn Chresten
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US05/487,368
• Time in Patent Office: 755 Days
• Field of Search: 356/36, 356/39, 356/40, 356/41, 356/73, 73/69, 259/DIG. 44
• US Class Current: 356/36
• CPC Class Codes: G01N 1/286 involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising microtomes G01N1/06; pulverising in general B02C; mixing in general B01F
• CPC Class Codes: G01N 2001/4094 using ultrasound
• CPC Class Codes: G02B 26/008 in the form of devices for effecting sequential colour changes, e.g. colour wheels

Abstract

The invention relates to a method and apparatus for measuring one or more data of blood samples, such as oxygen saturation, contents of hemoglobin, bicarbonate, sodium, potassium, etc. involving hemolyzation of the blood sample. The blood sample is passed into a conduit and hemolyzed therein by imparting ultra-sonic waves to all parts defining said conduits. Measurement is performed after and possibly also prior to hemolyzation. In its preferred embodiment the apparatus according to the invention comprises a computer for automatically controlling the operational sequence of the components of the apparatus and for receiving measuring signals from measuring devices of the apparatus and converting said signals into immediately understandable digital data.

Claims

1. An apparatus for hemolyzing a blood sample and for measuring at least one constituent thereof, said apparatus comprising:

a. a conduit for conducting said blood sample and including a hemolyzing section,

b. means for generating ultra-sonic waves in walls defining said hemolyzing section so as to at least partly hemolyze a blood sample contained therein,

c. means for measuring said constituent on the hemolyzed blood sample within said hemolyzing section, and

d. means for subsequently flushing the blood sample out of said conduit.

2. An apparatus according to claim 1, further comprising an anvil member, a block member, and an ultrasonic generator for vibrating said block member, said hemolyzing conduit section being positioned between and engaged by said anvil member and said block member.

3. An apparatus according to claim 2, further comprising means for resiliently mounting said anvil member.

4. An apparatus according to claim 1, wherein said haemolyzing conduit section comprises transparent wall parts, said measuring means comprising a photometric measuring device including a light source for emitting light through said transparent wall parts and through a blood sample positioned in said hemolyzing conduit section, a photoelectric cell for receiving light having passed through said transparent wall parts, and light filters positionable between said photoelectric cell and said transparent wall parts.

5. An apparatus according to claim 2, wherein said hemolyzing conduit section comprises transparent wall parts, said measuring means comprising a photometric measuring device including a light source for emitting light through said transparent wall parts and through a blood sample positioned in said hemolyzing conduit section, photoelectric cell for receiving light having passed through said transparent wall parts, and light filters positionable between said photoelectric cell and said transparent wall parts.

6. An apparatus according to claim 5, wherein a surface part of said block member engaging said hemolyzing conduit section defines a recess or pocket receiving said photoelectric cell therein, said anvil member having an opening permitting light from said light source to pass therethrough.

7. An apparatus according to claim 5, wherein said hemolyzing conduit section comprises a pair of flat, spaced, transparent panel members of a relatively hard material, and a frame member of a softer material sandwiched between the edge portions of said panel members.

8. An apparatus according to claim 7, wherein said panel members are of glass and said frame member is of hard plastic material.

9. An apparatus according to claim 6, further comprising means for maintaining said hemolyzing conduit section at a substantially constant temperature.

10. An apparatus according to claim 2, wherein said ultra-sonic generator is adapted to generate ultra-sonic waves at a frequency of 15 - 100 kilocycles per second.

11. An apparatus according to claim 4, further comprising electric means for controlling the following operations in timed relationship:

a. introducing said blood sample into said hemolyzing conduit section,

b. hemolyzing said blood sample by operating said ultra-sonic generator,

c. successively positioning at least two different of said light filters between said photoelectric cell and said transparent wall parts, and

d. flushing said blood sample out of said conduit, and a computer for receiving electrical signals from said photoelectric cell in response to light received by said photoelectric cell from said light source through said filters, and for displaying data for oxygen saturation of said blood sample calculated on the basis of said signals.

12. A method for measuring at least one solute in a sample of whole blood, said method comprising

a. passing the blood sample into a conduit,

b. thereafter at least partly hemolyzing said blood sample within a section of said conduit by means of ultra-sound,

c. measuring said solute in the hemolyzed blood sample in said conduit section, and

d. subsequently flushing said hemolyzed sample out of said conduit.

13. A method according to claim 12, wherein said blood sample is hemolyzed by ultrasound at a frequency of 15 - 100 kilocycles per second.

14. An apparatus for automatically measuring data of blood samples, said apparatus comprising:

a. a conduit for conducting a blood sample and including a hemolyzing section having transparent wall parts,

b. means for generating ultra-sonic waves in wall parts defining said hemolyzing section so as to at least partly hemolyze a blood sample contained therein,

c. a photometric measuring device including a light source for emitting light through said transparent wall parts and through a blood sample positioned therebetween, a photoelectric cell for receiving light having passed through said transparent wall parts and for generating electrical signals in response to the intensity of the light received, and light filters positionable between said photoelectric cell and said transparent wall parts,

d. means for moving the blood sample into said hemolyzing conduit section and for flushing said sample out of said conduit after hemolyzation and measurement thereof,

e. electronic control means for controlling the operation of the blood sample moving and flushing means, the ultra-sonic wave generating means, and the photometric measuring device in timed relationship, and

f. a computer for receiving said signals generated by said photoelectric cell and for transferring them into directly readable digital values.

15. An apparatus according to claim 14, wherein said means for generating ultra-sonic waves comprise an anvil member, a block member, and an ultra-high frequency generator for vibrating said block member, said hemolyzing conduit section being positioned between and engaged by said anvil member and said block member.

16. An apparatus according to claim 15, wherein a surface part of said block member engaging said hemolyzing conduit section defines a recess or pocket receiving said photoelectric cell therein, said anvil member having an opening permitting light from said light source to pass therethrough to said photoelectric cell.

17. An apparatus according to claim 15, wherein said haemolyzing conduit section comprises a pair of flat, spaced, transparent panel members of a relatively hard material, and a frame member of a softer material sandwiched between the edge portions of said panel members.

18. An apparatus according to claim 17, wherein said panel members are of glass, and said frame member is of a hard plastic material.

19. An apparatus according to claim 17, comprising means for maintaining said hemolyzing conduit section at a substantially constant temperature.

20. An apparatus according to claim 19, wherein said frequency generator is adapted to generate ultra-sonic waves at a frequency of 15 - 100 kilocycles per second.