Membrane for enzyme electrodes
First Claim
1. A polarographic cell structure for use in polarographic analysis of an unknown comprising an electrically insulating support means, and electrode means mounted in said support means, said electrode means including means defining an active exposed working face, wherein the improvement comprises:
- a laminated membrane of a total thickness of less than 25 microns positioned between said working face and said unknown, said membrane consisting essentially of a first layer of an essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate, and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an enzyme preparation positioned between and adhesively bonding said first and second layers.
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Abstract
A laminated membrane for use with an enzyme electrode without the need for use of a compensating electrode. The laminated membrane comprises a layer of essentially homogeneous material such as cellulose acetate or silicone rubber which will prevent passage of even low molecular weight interfering materials, an adhesive layer of the enzyme itself (with or without other materials that may be blended with it), and a layer of support film which will also prevent the passage of high molecular weight interfering materials.
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Citations
9 Claims
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1. A polarographic cell structure for use in polarographic analysis of an unknown comprising an electrically insulating support means, and electrode means mounted in said support means, said electrode means including means defining an active exposed working face, wherein the improvement comprises:
a laminated membrane of a total thickness of less than 25 microns positioned between said working face and said unknown, said membrane consisting essentially of a first layer of an essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate, and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an enzyme preparation positioned between and adhesively bonding said first and second layers. - View Dependent Claims (2, 3, 4)
- 5. A laminated membrane having a total thickness of less than 25 microns for use in a polarographic cell consisting essentially of a first layer of essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an enzyme preparation positioned between and adhesively bonding said first and second layers.
Specification