Test strips and methods and apparatus for using the same

• US 3,980,437 A
• Filed: 12/18/1975
• Issued: 09/14/1976
• Est. Priority Date: 12/21/1974
• Status: Expired due to Term

• Current Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Original Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Inventors: Yamamoto, Hiroshi, Kishimoto, Shinichi
• Primary Examiner(s): Reese, Robert M.
• Application Number:
  US05/641,829
• Time in Patent Office: 271 Days
• Field of Search: 23/253 TP, 356/199, 356/209
• US Class Current: 422/404
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00118 for multiple tests
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence

Abstract

A test strip of the type which is dipped into a liquid such as urine for indicating by color information with respect to the liquid. The test strip has a transparent backing on one side of which is located a color-reaction paper with a fastening layer situated between the paper and the backing for fastening the paper to the backing. The fastening layer is opaque and non-reflecting with respect to light, so that while light can pass freely through the backing it will reflected by the color-reaction paper without passing through the backing. The test strip is moved perpendicularly across an optical axis along which light is directed with the light traveling first through the transparent backing and then being reflected by the color-reaction paper. Signals are generated from the light passing through the backing and the light reflected by the color-reaction paper, with the timing of the signal from the reflected light being controlled by the signal from the light which has passed through the transparent backing. The signal from the light which has traveled through the backing is utilized to initiate a time interval after which the signal from the reflected light is generated so as to assure that the operation of the structure providing the signal from the reflected light takes place when the color-reaction paper intersects the optical axis.

Claims

1. A test strip of the type which, after being dipped in a liquid such as urine, will assume a color indicative of a property of the liquid, comprising a transparent backing through which light can freely pass, a color-reaction paper for assuming a given degree of a given color when detecting the intensity of a given property of a liquid in which the paper is immersed, said color-reaction paper being situated on one side of but being spaced from said backing, and fastening means situated between the paper and backing and fastening the paper to the backing, said fastening means having at least the same area as said paper and engaging the latter throughout its entire area which is directed toward the backing, and said fastening means being substantially opaque and non-reflecting with respect to light, so that light will pass freely through said backing at portions thereof not engaged by said fastening means while light will be reflected from said color-reaction paper while remaining substantially uninfluenced by said fastening means and backing.

2. The combination of claim 1 and wherein said fastening means has an area substantially equal to that of said paper.

3. The combination of claim 2 and wherein at least one additional color-reaction paper and one additional fastening means are carried by said backing with the additional fastening means situated between said additional paper and backing, said additional color-reaction paper having a standard color for providing a standard with respect to which the color of said first-mentioned color-reaction paper can be measured.

4. The combination of claim 2 and wherein a plurality of said color-reaction papers and a plurality of said fastening means are all carried by said backing, the latter being elongated and said plurality of papers and fastening means being distributed longitudinally along said backing while being spaced one from the next along said backing, the several color-reaction papers respectively indicating different properties with respect to a liquid in which all of the papers are dipped.

5. The combination of claim 2 and wherein said fastening means is a layer of adhesive material adhering on the one hand to said backing and on the other hand to said paper and has a color providing the layer of adhesive material with the substantially opaque, non-reflective properties with respect to light.

6. The combination of claim 5 and wherein the color of said adhesive layer is black.

7. In a method for utilizing a test strip composed of a transparent backing on the side of which there is a color-reaction paper fastened to the backing by a fastening means between the paper and backing which is substantially opaque and non-reflective with respect to liquid with respect to which the color-reaction paper will assume a given color, comprising the steps of directing light along a given optical axis and while maintaining the test strip perpendicular to said axis providing between the light and test strip a movement according to which the light passes first through the backing and then impinges on the color-reaction paper to be reflected thereby, generating a firt signal from the light which passes through said backing, generating from the light which reflects from said color-reaction paper a second signal indicative of the color of the reaction paper, and initiating the generation of said second signal at a given time interval after termination of said first signal by intersection of said color-reaction paper with said optical axis.

8. In a method as recited in claim 7 and including the step of generating a reference signal from a standard color-reaction paper prior to generation of said first and second signals, and then comparing said second signal with said reference signal for determining a property of the liquid.

9. In a method as recited in claim 7 and wherein a plurality of color-reaction papers respectively indicating different properties of a liquid in which they have previously been immersed are respectively fastened to the backing and distributed longitudinally along the latter by a plurality of the fastening means, respectively, which are substantially opaque and non-reflective with respect to light, and moving the test strip across the optical axis so that a plurality of said first signals respectively alternate with a plurality of second signals with each second signal being generated after the immediately preceding first signal after said given time interval.

10. In a method for utilizing a test strip having a transparent backing and a color-reaction paper situated on one side of said backing with a fastening means situated between the paper and backing for fastening the paper to the backing with the fastening means being substantially opaque and non-reflective with respect to light, the steps of directing light along a predetermined optical axis, moving the test strip perpendicularly across the optical axis with the backing first intersecting the optical axis and the color-reaction paper then intersecting the optical axis, so that light passes first freely through the backing and then is reflected by the color-reaction paper, generating a pair of signals first with light which has passed through the transparent backing and then with light which is reflected from the color-reaction paper, and utilizing the great difference between these signals for achieving a clear indication of the signal from light reflection by the color-reaction paper.

11. In an apparatus for utilizing a test strip having a transparent backing, a color-reaction paper on one side of the backing, and a fastening means between the paper and backing fastening the paper to the backing with the fastening means being substantially opaque and non-reflective with respect to light, optical means for directing light along a predetermined optical axis, transporting means connected with the test strip for transporting the latter perpendicularly across the optical axis in a manner permitting the light traveling along the optical axis first to pass freely through the backing and then to be reflected by the color-reaction paper, a pair of detecting means one of which is situated at the side of the test strip opposite from the optical means to receive light traveling through the transparent backing and the other of which is situated on the same side of the test strip as the optical means for receiving light reflected by the color-reaction paper, whereby each detecting means receives light sharply different from the light received by the other detecting means during an interval different from the interval during which the other detecting means receives light, and electrical means operatively connected with the pair of detecting means for responding to the sharp difference between the light received by the pair of detecting means for providing information with respect to the light reflected by the color-reaction paper.

12. The combination of claim 11 and wherein the backing is in the form of an elongated strip while a plurality of the color-reaction papers are distributed longitudinally along the strip, spaced from each other, with a plurality of fastening means respectively situated between the color-reaction papers and the strip fastening the papers to the strip, said transporting means including a pair of rolls on one of which the strip is initially wound and from which the strip is unwound while being wound up on the other of the rolls, with the rolls being situated on opposite sides of the optical axis for transporting the strip across the optical axis.

13. The combination of claim 11 and wherein said transporting means includes a carriage carrying the strip and formed in alignment therewith with an opening through which the light can freely pass after passing through the transparent backing to be received by said one detecting means.

14. The combination of claim 11 and wherein said electrical means includes a control means connected between said pair of detecting means for initiating operation of said other detecting means after said control means detects that the color-reaction paper has reached the optical axis by the reduction in the light received by said one detecting means.

15. The combination of claim 11 and wherein said electrical means includes a timer circuit means for initiating the operation of said transporting means only after the color-reaction paper has had sufficient time to react with respect to a liquid in which the color-reaction paper has previously been immersed.

16. The combination of claim 11 and wherein said electrical means includes a pair of detecting circuit means respectively connected with said pair of detecting means for converting light received thereby into predetermined voltages, and signal-discriminating circuit means connected between said pair of detecting circuit means for discriminating when the light received by said one detecting means has been diminished to an extent indicating that the color-reaction paper has reached the optical axis and for then operating the detecting circuit means connected to said other detecting means.

17. The combination of claim 16 and wherein a computing circuit means is connected with the detecting circuit means which is connected to said other detecting means for computing the signal generated from reflection of light from the color-reaction paper.

18. The combination of claim 17 and wherein said electrical means includes a memory circuit means connected between said computing circuit means and said detecting circuit means which is connected to said other detecting means for memorizing a reference signal to be compared with the signal provided by said detecting circuit means connected to said other detecting means at said computing circuit means, for providing information with respect to the light reflected from the color-reaction paper.