Automated chemical analyzer

• US 3,985,508 A
• Filed: 10/28/1975
• Issued: 10/12/1976
• Est. Priority Date: 10/28/1975
• Status: Expired due to Term

• Current Assignee: Melvin Williams
• Original Assignee: Melvin Williams
• Inventors: Williams, Melvin
• Primary Examiner(s): Reese, Robert M.
• Application Number:
  US05/626,053
• Time in Patent Office: 350 Days
• Field of Search: 23/253 R, 23/259
• US Class Current: 422/65
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes

Abstract

Apparatus for automatically performing multiple chemical analyses of a plurality of liquid specimens. The apparatus comprises apparatus for loading a plurality of test samples from each specimen into a row of reaction vessels. As the reaction vessels are transported to a determinating apparatus, other apparatus adds reagents to preselected ones of the reaction vessels a predetermined time before the reaction vessels reach the determinating apparatus. In particular, each of the several sample containers initially containing the specimens is transported in a track or the like in parallel with the one of the several rows of reaction vessels containing the test samples from the specimen toward the determinating apparatus. Microswitches engage each of the sample containers along the way, enabling the reagent adding apparatus to add selected reagents to the parallel row of reaction vessels. The reacted test samples are automatically analyzed by the determinating apparatus and the results output on a paper tape or the like. In addition, apparatus is provided for automatically removing the sample containers from the track after the determinations have been completed and depositing the containers in predetermined sequential order in a tray.

Claims

1. Apparatus for automatically performing multiple chemical analyses of a plurality of discrete liquid specimens comprising:

means comprising a plurality of sample containers, each of the sample containers initially containing one of the specimens;

means comprising a plurality of reaction vessels in each of several parallel aligned rows;

means for loading a plurality of specimen test samples from each of the sample containers into a corresponding one of the rows of reaction vessels, the loading means including means for automatically aspirating the plurality of test samples from each of the sample containers and means for sequentially conveying the sample containers to and from the aspirating means, the aspirating means discharging the plurality of test samples aspirated from the sample container into the corresponding row of reaction vessels before aspirating the test samples from the sample container subsequently conveyed to the aspiration means;

means for transporting the several rows of reaction vessels in a continuous sequence from the aspirating means to a determinating station,

the conveying means conveying each of the sample containers from the aspiration means in parallel with the corresponding row of reaction vessels containing the test samples aspirated from the sample container;

means comprising a plurality of switching devices being sequentially enabled by each of the sample containers being conveyed from the aspiration means for selectively adding reagents to certain ones of the reaction vessels, each of the switching devices being effective to cause one or more of the reagents to be added to selected ones of the reaction vessels in the row corresponding to the sample container enabling the switching device; and

means for automatically analyzing the reacted test samples at the determinating station and outputing the results of the analyses.

2. Apparatus in accordance with claim 1 wherein the aspiration means comprises a plurality of independently operable aspirating apparatus, each of the aspirating apparatus having an associated aspiration probe and being selectively actuable to aspirate one of the plurality of test samples from the sample container conveyed to the aspiration means and discharge the test sample into the corresponding reaction vessel, and wherein the conveying means includes means for transferring the sample container sequentially past the plurality of aspirating apparatus, the transfer means stopping at each aspirating apparatus while the test sample is aspirated from the sample container before proceeding to the next aspirating apparatus.

3. Apparatus in accordance with claim 1 including means for aspirating a portion of the specimen contained in the sample containers being conveyed from the aspiration means in parallel with the rows of reaction vessels being transported to the determinating station and flame photometer means for burning the aspirated specimen and monitoring the spectral characteristics data of the burning specimen, the flame photometer means coupling the spectral characteristics data to the analyzer means for outputing the data with the other results of the analyses of the reacted specimen test samples.

4. Apparatus in accordance with claim 1 including sample registration means for receiving the sample containers and means for removing the sample containers from the means conveying the sample containers from the aspiration means and placing the sample containers in the sample registration means in sequential order after the corresponding row of reaction vessels has reached the determinating station.

5. Apparatus in accordance with claim 1 wherein each of the aspirating apparatus includes means for positioning the associated aspiration probe, the positioning means inserting the probe into the specimen in the sample container to aspirate the sample therefrom and repositioning the probe to be over a corresponding one of the reaction vessels to discharge the test sample into the reaction vessel.

6. Apparatus in accordance with claim 5 wherein the aspirating apparatus include means for diluting the test samples and flushing the test sample from associated probes into the corresponding reaction vessels with a diluent, the diluent cleansing the probes in preparation for aspiration of the next test samples.

7. Apparatus in accordance with claim 1 wherein the reagent adding means comprises pump means having output means selectively positioned to add reagents to certain ones of the reaction vessels at predetermined times before the reaction vessels reach the determinating means as the rows of reaction vessels are transported from the aspiration means to the determinating station, the pump means being responsive to the switch devices to add reagents to certain ones of the reaction vessels in each row as the sample container corresponding to the row enables the switching device.

8. Apparatus in accordance with claim 7 wherein the reagent adding means comprises a plurality of the pump means, a plurality of multi-output distribution manifold means, each of the distribution manifold means being associated with a corresponding one of the switching devices and responsive thereto, and a plurality of pump manifold means, each of the pump manifold means having an input selectively coupled to one of the outputs of one of the distribution manifold means and a plurality of outputs selectively coupled to associated ones of the plurality of pump means, the output means of each of the pump means being positioned over certain ones of the reaction vessels in the row of reaction vessels corresponding to the switching device controlling the distribution manifold means coupled to the pump manifold means associated with the pump means, the distribution manifold means associated with any of the switching devices and any number of the pump manifold means being selectively intercoupled to provide a plurality of the pump means for adding reagents to the reaction vessels in the row of reaction vessels associated with the switching device.

9. Apparatus in accordance with claim 1 wherein the means for transporting the several rows of reaction vessels from the aspiration means to the determinating station and back to the aspiration means comprises a corresponding number of block means, each of the block means having cavities along the length thereof for holding one of the rows of reaction vessels, means for pushing the several block means in continuous sequence in direction normal to the lengths of the several block means from the aspiration means to the determination station, means for pushing the block means laterally at the determinating station after the reacted test samples have been analyzed, means for pushing the block means from the lateral position adjacent the determination station back to a position immediately adjacent and lateral to the position adjacent the aspiration means where the test samples are loaded into the reaction vessels, and means for pushing the block means laterally into the position adjacent the aspiration means.

10. Apparatus in accordance with claim 9 including a controlled temperature bed having selectively variable heating zones for supporting the block means and heating the test samples to required temperatures at various points between the aspiration means and the determinating station.

11. Apparatus in accordance with claim 1 wherein the reacted test sample analyzing means includes means for aspirating the reacted test samples from the reaction vessels at the determinating station and coupling the aspirated reacted test samples to the means for analyzing the reacted test samples.

12. Apparatus in accordance with claim 11 wherein the analyzing means comprises a lamp, a plurality of flow-through cuvettes coupled to the aspirating means, the reacted test samples flowing through corresponding ones of the cuvettes and each of the cuvettes being radially aligned with the lamp, and a plurality of spectrophotometer photocells for detecting the characteristics of the light passing through the reacted test samples in the cuvettes.

13. Apparatus in accordance with claim 12 wherein the lamp is a double filament lamp having first and second filaments and including light detector means for monitoring the light from the double filament lamp and enabling the second filament if the first filament stops emitting light.

14. Apparatus in accordance with claim 1 wherein the means for transporting the rows of reaction vessels from the aspiration means to the determinating station includes means for transporting the several rows of reaction vessels in a continuous sequence from the determinating station back to the aspiration means.

15. Apparatus in accordance with claim 14 including means for washing the reaction vessels with a washing liquid as the rows of reaction vessels are transported back to the aspiration means from the determinating station to receive other test samples.

16. Apparatus in accordance with claim 15 wherein the washing means comprises a plurality of tubes, a block having a corresponding plurality of cavities therein, means for covering the block so that a small air space exists between the cover means and the block with which the plurality of cavities communicate, each one of the plurality of tubes extending through the cover means into a corresponding one of the cavities, means for pumping a washing liquid into the air space between the block and the cover means to force the washing liquid into the cavities and out the tubes, and means for positioning the tubes over the plurality of reaction vessels to wash the reaction vessels as the rows of reaction vessels are transported back to the aspiration means from the determinating station.

17. Apparatus in accordance with claim 15 including means for aspirating the washing liquid from the reaction vessels.

18. Apparatus in accordance with claim 1 wherein the conveying means includes a track for sequentially delivering the sample containers to the transfer means one at a time for transfer past the aspiration apparatus and a track for returning the sample container from the transfer means after the plurality of test samples have been aspirated therefrom, the sample container being conveyed in the return track in parallel with the row of reaction vessels containing the test samples aspirated from the sample container.

19. Apparatus in accordance with claim 18 wherein the transfer means comprises trolley means for carrying the sample container and reversing motive means for moving the trolley means from the delivery track, to the aspiration means, past the plurality of aspirating apparatus, the motive means there reversing to move the trolley means back to the delivery track, the trolley means pausing at the return track while the sample container is moved from the trolley means into the return track.

20. Apparatus in accordance with claim 18 wherein the conveying means includes an end track interconnecting the other ends of the delivery track and the return track and means for moving the sample containers along the tracks to and from the transfer means in a continuous sequence.

21. Apparatus in accordance with claim 20 including receptacles for carrying the sample containers in the tracks and the transfer means, adjacent ones of the receptacles abutting each other in the tracks in a continuous sequence and wherein the means for moving the sample containers along the tracks comprises pneumatic means aligned with the ends of the return track, the end track and the delivery track, the pneumatic means aligned with the delivery track pushing the receptacles along the delivery track to load the receptacle at the other end of the track into the transfer means, the pneumatic means aligned with the end track pushing a receptacle at the other end of the end track into the delivery track at the position adjacent the delivery track pneumatic means, and the pneumatic means aligned with the return track subsequently pushing the receptacle previously loaded into the transfer means from the transfer means into the return track and causing the receptacle at the other end of the return track to be pushed into position in the end track adjacent the end track pneumatic means.

22. Apparatus in accordance with claim 21 including means comprising a tray having a plurality of cavities therein arranged in X-Y grid pattern in arbitrarily designated X and Y directions and including X-Y positioning means for initially positioning the pick-up means at a pick-up position over the means conveying the sample containers from the aspiration means to pick up one of the sample containers the corresponding receptacle and for repositioning the pick-up means over one of the cavities in the tray whereupon the pick-up means releases the sample container into the cavity, the X-Y positioning means directing the sample containers received sequentially from the conveying means to the cavities in the X-Y grid pattern in sequential order.