Test composition, device, and method for the detection of peroxidatively active substances
First Claim
1. A test composition for detection of peroxidatively active substances in body fluids, body excreta and the like, of the type including an indicator capable of being oxidized in the presence of a peroxidatively active substance to provide a color change, an oxidizing agent effective to oxidize said indicator, and a potentiating agent, wherein said potentiating agent comprises a water soluble acid salt or adduct of a compound selected from the class of compounds having the formula ##SPC3##wherein R1 is hydrogen, lower alkyl, hydroxy or thiophenyl;
- R2 is hydrogen or lower alkyl;
R3 is hydrogen or lower alkyl;
R4 is hydrogen, carboxy, lower alkyl or lower alkoxy; and
R5 is hydrogen or lower alkyl.
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Abstract
Improved test compositions, devices and methods are provided for detecting peroxidatively active substances in body fluids, excreta and the like. In the type of test compositions including an indicator capable of being oxidized in the presence of a peroxidatively active substance to provide a color change, an oxidizing agent effective to oxidize said indicator, and a potentiating agent, the improvement comprises the use as said potentiating agent of an acid salt or adduct of a compound selected from the class of compounds of the general formula ##SPC1##
Wherein R1 is hydrogen, lower alkyl, hydroxy or thiophenyl; R2 is hydrogen or lower alkyl; R3 is hydrogen or lower alkyl; R4 is hydrogen, carboxy, lower alkyl or lower alkoxy; and R5 is hydrogen or lower alkyl.
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Citations
19 Claims
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1. A test composition for detection of peroxidatively active substances in body fluids, body excreta and the like, of the type including an indicator capable of being oxidized in the presence of a peroxidatively active substance to provide a color change, an oxidizing agent effective to oxidize said indicator, and a potentiating agent, wherein said potentiating agent comprises a water soluble acid salt or adduct of a compound selected from the class of compounds having the formula ##SPC3##
wherein R1 is hydrogen, lower alkyl, hydroxy or thiophenyl; - R2 is hydrogen or lower alkyl;
R3 is hydrogen or lower alkyl;
R4 is hydrogen, carboxy, lower alkyl or lower alkoxy; and
R5 is hydrogen or lower alkyl. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
- R2 is hydrogen or lower alkyl;
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17. A test device for detecting peroxidatively active substances in body fluids, excreta and the like, of the type having a carrier matrix incorporated with an oxidizing agent, an indicator, and a potentiating agent wherein said potentiating agent comprises a water soluble acid salt or adduct of a compound selected from the class of compounds of the general formula ##SPC4##
wherein R1 is hydrogen, lower alkyl, hydroxy or thiophenyl; - R2 is hydrogen or lower alkyl;
R3 is hydrogen or lower alkyl;
R4 is hydrogen, carboxy, lower alkyl or lower alkoxy; and
R5 is hydrogen or lower alkyl. - View Dependent Claims (18, 19)
- R2 is hydrogen or lower alkyl;
Specification