Separation of cells from liquid components of blood

• US 3,990,849 A
• Filed: 02/07/1975
• Issued: 11/09/1976
• Est. Priority Date: 02/07/1975
• Status: Expired due to Term

• Current Assignee: Technicon Instruments Corporation (Bayer AG)
• Original Assignee: Technicon Instruments Corporation (Bayer AG)
• Inventors: Oberhardt, Bruce J., Lee, Martin J.
• Primary Examiner(s): Reese, Robert M.
• Application Number:
  US05/548,177
• Time in Patent Office: 641 Days
• Field of Search: 23/230 B, 23/283 R, 210/DIG. 23, 210/39, 210/40, 210/41, 210/516, 356/36, 356/39, 356/246, 195/103.5
• US Class Current: 436/169
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: Y10S 68/90 Foam treatment
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A method for separating a blood cell portion and a liquid portion of a whole blood sample comprising the steps of contacting over a period of time a substance in the form of a nonparticulate gel mass with the sample, so as to diffuse the liquid portion into the mass, the mass having an effective pore size to exclude the blood cells, and collecting the blood cells remaining after such contact of the sample with the mass. The method may include impregnating the substance of the mass with a selective reagent to react with the liquid portion diffused therein, and furthr include analyzing a reaction product produced within the mass.

Claims

1. A method for separating a blood cell portion and a liquid portion of a whole blood sample, comprising the steps of:

contacting over a period of time a substance in the form of a nonparticulate gel mass with said whole blood sample, so as to diffuse the liquid portion thereof into said gel mass, said gel mass having an effective pore size to exclude the blood cell portion, and collecting the blood cell portion remaining after contact of said sample with said gel mass.

2. A method as defined in claim 1, further including the step of holding said sample in a stagnant, captive condition while the latter is in contact with said gel mass.

3. A method as defined in claim 1, further including impregnating said substance with a selective reagent to react with said liquid sample portion when the latter is diffused in said gel mass.

4. A method as defined in claim 3, further including the step of analyzing the product produced within said gel mass on reaction of said reagent and said sample portion.

5. A method as defined in claim 1, further including forming a well in said gel mass, said collecting of the blood cell portion comprising collecting said cell portion in said well, and said contacting of said gel mass with said sample comprising introducing said sample into said well.

6. A method as defined, in claim 5, further including impregnating said substance with a selective reagent to react with said liquid sample portion when the latter is diffused in said gel mass and photometrically analyzing the reaction product produced within said gel mass by directing a beam of light through said gel mass.

7. A method as defined in claim 1, further including supporting plural ones of said gel masses in separated condition from one another, impregnating each substance forming one of said gel masses with a reagent, to react with a diffused part of said sample liquid portion said contacting step comprising contacting all said gel masses with said sample for diffusion into the respective gel masses of different parts of said sample liquid portion.

8. A method as defined in claim 7, wherein:

said step of supporting said gel masses comprises stacking said gel masses with moisture-impervious barriers therebetween, said impregnating step comprises impregnating the substance of each gel mass with a different reagent to react with a part of the sample liquid portion when the latter is diffused thereinto, and analyzing the different reaction products in all said gel masses.

9. A method as defined in claim 9, wherein:

said analyzing step includes concurrently analyzing all said reaction products.

10. An article, comprising:

a substrate, means supported from said substrate for separating a whole blood sample into a cell portion and a liquid portion when contacted over a period of time with said sample, said means comprising a nonparticulate gel mass for diffusion thereinto of said liquid portion, said gel mass having an effective pore size to exclude said cell portion, and a selected reagent in said gel mass to react with said sample portion when the latter is diffused into said gel mass, said gel mass defining a sample-receiving well therein, said substrate comprising an upright cuvette within which said gel mass is vertically supported and further including a moisture-impervious barrier above said gel mass in abutment therewith and located a distance below the top of said cuvette, said barrier defining a vertical opening therethrough forming a part of said well, said cuvette and said barrier defining a well enlargement.

11. An article as defined in claim 10, wherein:

said means comprises plural ones of said gel masses supported in separated condition from one another for contact with respective parts of said sample, and further including a different reagent in each gel mass for reaction with a different part of said sample liquid portion diffused in said mass.

12. An article as defined in claim 10, wherein:

said means comprises plural ones of said gel masses supported in separated condition from one another for contact with respective parts of said sample, said gel masses being in vertically stacked relation with moisture-impervious barriers therebetween, said gel masses and said barriers defining a vertical common sample-receiving well.

13. An article as defined in claim 12, further including a moisture-impervious barrier directly above the uppermost one of said gel masses and located a distance below the top of said cuvette, the last-named barrier defining an opening forming a part of said common well, said cuvette and the last-named barrier defining a well enlargement.