Method of determining concentration of luteinizing hormone in body fluid

• US 3,991,174 A
• Filed: 04/17/1975
• Issued: 11/09/1976
• Est. Priority Date: 07/20/1970
• Status: Expired due to Term

• Current Assignee: RAFA Laboratories Limited
• Original Assignee: RAFA Laboratories Limited
• Inventors: Grundman, Lea
• Primary Examiner(s): Thomas, Jr., James O.
• Assistant Examiner(s): Fagelson, A. P.
• Application Number:
  US05/568,857
• Time in Patent Office: 572 Days
• Field of Search: 424/8, 424/12, 424/99, 424/100, 424/105
• US Class Current: 436/521
• CPC Class Codes: G01N 33/76 Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
• CPC Class Codes: Y10S 436/814 Pregnancy
• CPC Class Codes: Y10S 436/817 Steroids or hormones
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin
• CPC Class Codes: Y10S 436/826 Additives, e.g. buffers, diluents, preservatives

Abstract

The invention relates to a method of determining the concentration of luteinizing hormone (hereinafter referred to as "LH") in the body fluid of a woman and thereby determining the day when said woman ovulates, as well as to compositions for effecting this determination. The composition comprises human chorionic gonadotropin (hereinafter referred to as "HCG") coupled to red blood cells by means of glutaraldehyde as coupling agent therebetween, which composition in carrying out the test is mixed with the body fluid of the woman and an anti-HCG serum in an amount adjusted to react with approximately 50 international units (I.U.) of HCG per liter of the body fluid, generally urine, so that agglutination occurs when LH equivalent to less than 50 I.U. of HCG/L is present and no agglutination when at least 50 I.U. HCG/L is present. The day of highest concentration of LG equivalent to HCG indicating the day of ovulation.

Claims

1. Method for determining the concentration of luteinizing hormone in the body fluid of a woman, which comprises reacting the body fluid of the woman with a predetermined amount of anti-human chorionic gonadotropin and with a composition of human chorionic gonadotropin coupled to red blood cells by means of glutaraldehyde and also containing a stabilizing effective amount of gelatin, whereby hemagglutination occurs when said body fluid contains an amount of luteinizing hormone less than the amount which will react with all of said anti-human chorionic gonadotropin and no hemagglutination occurs when the amount of said luteinizing hormone in said body fluid is greater than the amount which will react with all of said predetermined amount of anti-human chorionic gonadotropin.

2. The method of claim 1 wherein said body fluid is urine.

3. The method of claim 2 wherein daily samples of filtered urine from said woman are mixed with predetermined amounts of anti-human chorionic gonadotropin and permitted to stand for about one hour, after which predetermined amounts of said human chorionic gonadotropin coupled to red blood cells by means of glutaraldehyde are added to said mixture which are then permitted to stand and the samples are examined to determine the relative concentrations of luteinizing hormone therein, the sample indicating the maximum concentration of luteinizing hormone in said body fluid being that of the day of ovulation of the woman.

4. The method of claim 1 wherein said predetermined amount of anti-human chorionic gonadotropin is selected as the amount which will react with the equivalent of up to 50 international units per liter of chorinic gonadotropin.

5. The method of claim 3 wherein said predetermined amount of anti-human chorionic gonadotropin is selected as the amount which will react with luteinizing hormone in an amount which is the equivalent of up to 50 international units of human chorionic gonadotropin per liter of body fluid.

6. The method of claim 3 wherein the test sensitivity is increased by diluting the urine.