Ultrafiltration process for obtaining protein isolates of vegetable origin

• US 3,993,636 A
• Filed: 09/13/1974
• Issued: 11/23/1976
• Est. Priority Date: 09/14/1973
• Status: Expired due to Term

• Current Assignee: Institut National de La Recherche Agronomique
• Original Assignee: Institut National de La Recherche Agronomique
• Inventors: Chopin, Marie-Christine, Maubois, Jean-Louis Joseph, Chopin, Alain, Culioli, Joseph
• Primary Examiner(s): Schain, Howard E.
• Application Number:
  US05/505,912
• Time in Patent Office: 802 Days
• Field of Search: 260/123.5
• US Class Current: 530/377
• CPC Class Codes: A23J 1/14 from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
• CPC Class Codes: B01D 61/145 Ultrafiltration
• CPC Class Codes: B01D 63/02 Hollow fibre modules

Abstract

An easily extrudable protein can be obtained from a vegetable source, such as sunflowers, by treating an alkaline solution of the protein obtained from said source, to ultrafiltration, through a semi-permeable membrane, having a pore size of 0.1 to 30 mμ, and adding a wash liquid to said solution while continuing said ultrafiltration.

Claims

1. A process for obtaining a purified and concentrated isolate of a vegetable source which comprises

dissolving the ground seeds, flour or meal from sunflower or colza in an alkaline solution, thereby forming an alkaline protein solution;

subjecting said solution to ultrafiltration with a semipermeable membrane having a pore diameter of 0.1 to 30 mμ

at a temperature of 2°

-30°

C, to form a retentate having an amount of nitrogenous matter (N ×

6.25) in the range of 3 to 12% by weight of the retentate;

adding to said retentate a volume of wash liquid while continuing ultrafiltration at a temperature of 2°

-30°

C, such that when the volume of the permeate is equal to said added volume, the concentration of nitrogenous matter (N ×

6.25) in the retentate will be 70 to 85% by weight of the dry matter of said retentate;

adjusting the temperature of said retentate to 20°

-60°

C and continuing said ultrafiltration procedure; and

thereafter recovering said concentrated and purified retentate.

2. The process of claim 1, wherein wash liquid used is water, or an alkaline extraction solution having a pH substantially the same as that of the retentate.

3. The process of claim 1 wherein said wash liquid water is added immediately after contacting the alkaline protein solution with the semipermeable membrane.

4. A process of claim 1 wherein said vegetable protein source is sunflower meal which is prepared by the process which comprises:

a. suspending a ground sunflower meal at a temperature of from 20°

to 50°

C, in an alkaline solution, the amount of alkaline solution used being 8 to 12 times the weight of the meal, the alkalinity of the said solution being such that the pH of the resulting mixture is between 8 and 12;

b. permitting the mixture obtained in (a) to macerate while stirring for 20 to 60 minutes, removing the insoluble residue to form a clarified alkaline solution;

c. contacting the clarified alkaline solution with a semipermeable membrane at a temperature in the range of 2°

to 30°

C, the pore diameter of said membrane being in the range of 0.1 to 30 mμ

, the liquid entering the ultrafiltration module being at a pressure in the range of 1 to 50 kg/cm² ;

d. repeating step (c) until the level of nitrogenous matter (N ×

6.25) in said retentate is in the range of 3 to 12%;

e. continuing ultrafiltration at a temperature in the range of 2°

to 30°

C, and adding a volume of water or alkaline solution having a pH substantially the same as that of the retentate, whereby when the volume of liquid which has passed through said membrane is equal to said added volume, the concentration of nitrogenous matter (N ×

6.25) in the retentate amounts to 70 to 85% of the total dry matter;

f. continuing ultrafiltration of said retentate at a temperature in the range of 20°

to 60°

C, using the said semipermeable membrane until the concentration of nitrogenous matter in the final retentate is between 85 to 95% of the total dry matter.

5. The process of claim 4, wherein the sunflower meal is suspended in an aqueous sodium sulphite containing solution and wherein during step (b) the pH is adjusted by the use of an alkaline solution to a value of between 8 and 12.

6. The process of claim 5, wherein a sodium or potassium hydroxide solution having a concentration in the range of 0.1 to 10g/l, is used as the alkaline solution.

7. The process of claim 1, wherein said semipermeable membrane has a pore diameter in the range of 0.1 to 30mμ

, and a cut-off in the range of 2,000 to 30,000.

8. The process of claim 1, wherein the liquid is passed at right angles to the membrane at a velocity of about 1 meter/second, and wherein the solution entering the ultrafiltration module is at a pressure in the range of 1 to 50kg/cm².

9. The process of claim 1, wherein the final retentate is, further, dried by freeze-drying or spray-drying or frozen at between -20°

C to -80°

C.

10. The process of claim 1, wherein the proteins of vegetable origin are isolated in their insoluble form by precipitation, adjusting the pH of the final retentate to the isoelectric pH of the protein, after which the proteins so precipitated are washed and dried.