Apparatus for automatic application of blood serum

• US 3,999,505 A
• Filed: 06/03/1975
• Issued: 12/28/1976
• Est. Priority Date: 06/08/1974
• Status: Expired due to Term

• Current Assignee: Olympus Optical Corporation Limited (Olympus Corporation)
• Original Assignee: Olympus Optical Corporation Limited (Olympus Corporation)
• Inventors: Kato, Yutaka, Ouchi, Teruo, Tokitoh, Hirohiko
• Primary Examiner(s): McIntosh, John P.
• Application Number:
  US05/583,266
• Time in Patent Office: 574 Days
• Field of Search: 118/242, 118/7, 118/243, 118/263, 118/73, 118/DIG. 23, 118/203, 118/104, 118/224, 118/249, 118/401, 354/318, 23/253 R, 23/230 B, 23/258.5
• US Class Current: 118/704
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01N 35/00009 provided with a sample supporting tape, e.g. with absorbent zones

Abstract

An apparatus for automatic application of blood serum comprises an applicator, a rinsing unit and a drip both associated with the applicator, means for conveying a blood serum bearing film while maintaining it in opposing relationship with the applicator, and means for supplying a buffer solution for wetting the film with a buffer solution prior to the application of a blood serum thereto. Blood serum is automaticaly applied to the film which is previously wetted with the buffer solution in an automatic manner.

Claims

1. An apparatus for automatic application of blood serum comprising guide means, a serum applicator movable along the guide means;

drive means for moving the applicator to a serum supply station, a serum application station and a rinsing station in sequence, said stations being arranged at closely spaced intervals and adjacent to the guide means, and a dripping device associated with the applicator and positioned adjacent to at least one of the stations, the dripping device including a paper filter for removing by absorption of rinsing water adhering to the applicator, and at least one roller disposed for contact with at least one surface of the paper filter for causing a movement thereof, said dripping device being effective to contact the applicator which has been subjected to an operation in the rinsing station by bringing a forward end of the applicator into abutment against the paper filter, said paper filter being moved by a given distance by rotating the roller for a limited time interval during the movement of the applicator through the various stations, thereby presenting a fresh portion of the paper filter for abutment by the applicator.

2. Automated apparatus for applying a measured sample of serum to a substrate for subsequent testing comprising:

means for applying a buffering solution to said substrate;

applicator means;

a serum supply station;

an application station;

said supply and application stations being arranged at closely spaced intervals;

means for moving the buffered substrate from said buffer applying means to said application station;

guide means positioned above said stations and adapted to guide the applicator means past all of the stations;

means for sequentially moving said applicator means along said guide means, which define a first linear path, to said serum supply station and said application station;

means responsive to positioning of the applicator means above each station for halting the applicator means; and

means responsive to halting of the applicator means for reciprocally moving said applicator means in a direction transverse to said first linear path to sequentially enable the applicator means to pick up a measured amount of serum at the supply station and deposit said serum upon said substrate at the application station.

3. The apparatus of claim 2 wherein said buffering solution applying means includes a pair of rollers disposed in abutting relationship with each other, at least one of the rollers having its surface portion formed of a sponge material, the device also including a hollow shaft which carries said one roller and formed with a plurality of apertures for permitting penetration of a buffering solution into said one roller through the hollow shaft, said rollers operating to feed a serum bearing film through the nip therebetween and to wet it with the buffering solution which has penetrated through said one roller, the surface portion of said one roller being effective to remove by absorption an excess amount of buffering solution from the film as the rollers are disengaged from their abutting relationship.

4. The apparatus of claim 2, further comprising a rinsing station containing a rinsing solution and arranged at a closely spaced interval relative to one of said supply and application stations;

said application moving means further comprising means for sequentially moving said applicator means along said first linear path from said application station to said rinsing station;

said halting means being further adapted to halt the applicator means when positioned above the rinsing station; and

said reciprocating means further being adapted to move said applicator means into and out of said rinsing solution when the applicator means is halted at the rinsing station.

5. The apparatus of claim 4, further comprising a drip station;

said drip station comprising means for incrementally moving an elongated absorbing substrate along a second linear path;

said applicator moving means further comprising means for moving said applicator means along said first linear path from said rinsing station to said drip station;

means responsive to movement of the applicator means adjacent the drip station to halt said applicator means;

said reciprocating means including means responsive to halting of the applicator means and adapted to move said applicator means into and out of engagement with said absorbing substrate to dry said applicator means preparatory to the next serum application and rinsing operations whereby said incremental moving means advances an unused portion of said absorbing substrate into position for the next drying operation.

6. The apparatus of claim 2, wherein said buffering solution applying means comprises a pair of rollers disposed in abutting relationship with each other, at least one of the rollers having its surface portion formed of a sponge material, said one roller being maintained immersed in a supply of buffering solution, said pair of rollers operating to feed a serum bearing surface through the nip therebetween while wetting it with the buffering solution, the surface portion of said one roller being effective to remove by absorption an excess amount of buffering solution from the film as the rollers are disengaged from their abutting relationship.