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Method and apparatus for sealed, sterile connection

  • US 4,004,586 A
  • Filed: 03/12/1975
  • Issued: 01/25/1977
  • Est. Priority Date: 03/12/1975
  • Status: Expired due to Term
First Claim
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1. The method of transferring sterile contents from a first, sealed, sterile container, having a first, flexible, sealed, fluid transfer tube, to a second, sealed, sterile container having a second, flexible, sealed, fluid transfer tube, said first and second tubes communicating at one end with their respective sealed, sterile containers, said first and second tubes having a free end, and being each sealed with a diaphragm across their bores, said diaphragms being spaced from the free ends of said first and second tubes, said method comprising:

  • inserting one end of a double-ended, hollow spike into each of the bores of said first and second tubes at the free end thereof, said spike being proportioned to tightly and sealingly fit into each bore of the first and second tubes;

    sterilizing said spike and that portion of said first and second tubes extending from each said diaphragm to the free ends of said tubes; and

    thereafteraxially collapsing said first and second tubes adjacent the free ends thereof to pass the ends of said spike through said diaphragms to open a flow channel between said first and second containers;

    transferring said sterile contents through said fluid transfer tubes from said first to said second container; and

    resealing said second fluid transfer tube while sterility is retained in said second tube.

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