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Preparation of active proteins cross-linked to inactive proteins

  • US 4,004,979 A
  • Filed: 01/15/1975
  • Issued: 01/25/1977
  • Est. Priority Date: 03/29/1968
  • Status: Expired due to Term
First Claim
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1. A process for producing an article containing a cross-linked active protein substance which comprises dispersing, mixing and reacting in a solvent medium 1. an active protein and 2. an inactive protein simultaneously with 3. a cross-linking agent for said proteins under cross-linking conditions for said cross-linking agent until an active protein substance is formed containing said active protein cross-linked with said inactive protein substance, controlling the degree of cross-linking by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth, said formed active protein substance amounting to about 1 to 20% by weight of the total proteinic substances and said cross-linking agent amounting to about 0.5 to 8% weight based on the weight of the whole mixture being reacted and separating the solvent from said formed active protein substance.

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