Preparation of active proteins cross-linked to inactive proteins
First Claim
1. A process for producing an article containing a cross-linked active protein substance which comprises dispersing, mixing and reacting in a solvent medium 1. an active protein and 2. an inactive protein simultaneously with 3. a cross-linking agent for said proteins under cross-linking conditions for said cross-linking agent until an active protein substance is formed containing said active protein cross-linked with said inactive protein substance, controlling the degree of cross-linking by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth, said formed active protein substance amounting to about 1 to 20% by weight of the total proteinic substances and said cross-linking agent amounting to about 0.5 to 8% weight based on the weight of the whole mixture being reacted and separating the solvent from said formed active protein substance.
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Abstract
Articles containing active proteins, such as enzymes, crosslinked to an inactive protein are prepared by dispersing and reacting in a solvent an active protein and an inactive protein simultaneously with a crosslinking agent to crosslink the active and inactive proteins together. The degree of crosslinking can be controlled by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth. Optionally, an inert carrier may be present during crosslinking. The resultant crosslinked active and inactive proteins can be in various forms such as solutions, suspensions, gels, films, membranes, granules, pills or tablets.
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3 Claims
- 1. A process for producing an article containing a cross-linked active protein substance which comprises dispersing, mixing and reacting in a solvent medium 1. an active protein and 2. an inactive protein simultaneously with 3. a cross-linking agent for said proteins under cross-linking conditions for said cross-linking agent until an active protein substance is formed containing said active protein cross-linked with said inactive protein substance, controlling the degree of cross-linking by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth, said formed active protein substance amounting to about 1 to 20% by weight of the total proteinic substances and said cross-linking agent amounting to about 0.5 to 8% weight based on the weight of the whole mixture being reacted and separating the solvent from said formed active protein substance.
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