Enzyme entrappment with cellulose acetate formulations

• US 4,004,980 A
• Filed: 03/25/1975
• Issued: 01/25/1977
• Est. Priority Date: 03/25/1975
• Status: Expired due to Term

• Current Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Original Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Inventors: Lim, Henry C., Emery, Alden H., Kolarik, Michael J.
• Primary Examiner(s): Naff, David M.
• Application Number:
  US05/561,685
• Time in Patent Office: 672 Days
• Field of Search: 195/63, 195/68, 195/DIG. 4, 106/196, 264/200, 264/207, 260/227, 260/230
• US Class Current: 435/179
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres
• CPC Class Codes: C12N 11/12 Cellulose or derivatives thereof
• CPC Class Codes: C12P 19/24 produced by the action of an isomerase, e.g. fructose
• CPC Class Codes: D01F 1/02 Addition of substances to the spinning solution or to the melt addition of substances to viscose D01F2/08

Abstract

Enzymes are entrapped by preparing a polymer formulation containing cellulose triacetate and cellulose diacetate or glucose pentaacetate, adding an enzyme to the formulation, extruding the enzyme-containing formulation to form a solid matrix and soaking the solid matrix in an aqueous solution of acetone to swell and increase the permeability of the solid matrix.

Claims

1. A method of forming an entrapped enzyme, said method comprising:

providing cellulose triacetate in solid form;

adding a second solid material to said cellulose triacetate, said second solid material being selected from the group consisting of cellulose diacetate and glucose pentaacetate;

dissolving the cellulose triacetate and second solid material in a solvent;

adding an enzyme to said cellulose triacetate and second solid material;

extruding said dissolved cellulose triacetate and said second solid material through an orifice to form a solid matrix soluble in a swelling liquid and

soaking said solid matrix in an aqueous solution of acetone and water containing between about 50 to 98% acetone for swelling said matrix to increase the permeability of the solid matrix.

2. The method of claim 1 wherein said second material is glucose pentaacetate and is added in solid form to said cellulose triacetate, said glucose pentaacetate being in the range of between 20 and 50% of said cellulose triacetate.

3. The method of claim 1 wherein said second material is cellulose diacetate and is added in solid form to said cellulose triacetate, said cellulose diacetate being in the range of between 20 and 80% of said cellulose triacetate.

4. The method of claim 1 wherein said cellulose triacetate and said second solid material are dissolved in methylene dichloride.

5. The method of claim 1 wherein said dissolved cellulose triacetate and second solid material are spun through an orifice to form a fiber.

6. The method of claim 1 wherein said dissolved cellulose triacetate and second solid material are cast through an orifice to form a film.

7. The method of claim 1 wherein said solid matrix is soaked in a mixture of acetone and water containing about 50% acetone.

8. A method of forming an entrapped enzyme, said method comprising:

providing a mixture in liquid form of cellulose triacetate and a second material selected from the group consisting of cellulose diacetate and glucose pentaacetate with both said cellulose triacetate and said second material having been dissolved from solid form in solvent;

adding an enzyme to said cellulose triacetate and second solid material;

extruding said dissolved cellulose triacetate and said second solid material through an orifice to form a solid matrix soluble in a swelling liquid; and

soaking said solid matrix in an aqueous solution of acetone and water containing between about 50 and 98% acetone for swelling said matrix to increase the permeability of the solid matrix.