Support for electrophoretic analysis

• US 4,006,069 A
• Filed: 11/17/1975
• Issued: 02/01/1977
• Est. Priority Date: 11/15/1974
• Status: Expired due to Term

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Yaginuma, Nakatsugu, Hiratsuka, Nobuo
• Primary Examiner(s): Prescott, Arthur C.
• Application Number:
  US05/632,884
• Time in Patent Office: 442 Days
• Field of Search: 204/180 G, 204/299, 204/230 B, 204/253 R
• US Class Current: 204/456
• CPC Class Codes: B01D 69/1071 Woven, non-woven or net mesh
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture

Abstract

A support for use in electrophoretic analysis comprising a porous polymeric flat plate such as filter paper, cellulose acetate or a non-woven fabric and a polymeric gel, such as polyacrylamide gel, enclosed in the open cells of the flat plate. The gel membrane is tough and does not shrink upon drying. The support can also be used as a filter.

Claims

1. In an electrophoretic analysis process utilizing a supported analysis member, the improvement wherein said supported analysis member comprises a porous polymeric flat plate and a polymeric gel enclosed in the open cells of the flat plate.

6. The support of claim 1, wherein the pore size of the flat plate ranges from 0.1 to 100 μ

.

2. A method for producing a support for electrophoretic analysis which comprises enclosing a sol in the pores of a porous polymeric flat plate and thereafter gelling the same.

3. A method for producing a support for electrophoretic analysis which comprises enclosing a monomer in the pores of a porous polymeric flat plate and thereafter polymerizing the same to form a gel.

4. A support for electrophoretic analysis comprising a porous polymeric flat plate and a polymeric gel enclosed in the open cells of the flat plate.

5. The support of claim 4, wherein the flat plate has a porosity of about 10 to about 90%.

7. The support of claim 4, wherein the flat plate is a cellulose acetate membrane or non-woven fabric membrane.

8. The support of claim 5, wherein the flat plate is a cellulose acetate of a non-woven fabric having a porosity of 50 to 90%.

9. The support of claim 5, wherein the flat plate is a filter paper having a porosity of 10 to 60%.

10. The support of claim 7, wherein the cellulose acetate membrane has a pore size of 0.1 to 10 microns.

11. The support of claim 4, wherein the polymeric gel is a polyacrylamide gel.

12. The support of claim 4, wherein the polymeric gel is an agar gel.

13. The support of claim 4, wherein the polymeric gel is a starch gel.

14. A filter consisting of a porous polymeric flat plate and a polymeric gel sealed in the open cells of the flat plate.

15. The filter of claim 14, wherein the porous polymeric flat plate is a cellulose acetate membrane.