Reagent supply control in automated fluid analysis

• US 4,009,999 A
• Filed: 05/29/1975
• Issued: 03/01/1977
• Est. Priority Date: 05/29/1975
• Status: Expired due to Term

• Current Assignee: Technicon Instruments Corporation (Bayer AG)
• Original Assignee: Technicon Instruments Corporation (Bayer AG)
• Inventors: Negersmith, Kent M.
• Primary Examiner(s): Scovronek, Joseph
• Application Number:
  US05/582,059
• Time in Patent Office: 642 Days
• Field of Search: 23/230 R, 23/230 B, 23/253 R, 23/259
• US Class Current: 436/53
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream

Abstract

Apparatus and method for analyzing successive liquid samples and conserving reagent. The method includes flowing a stream of discrete samples along a conduit from a source, which conduit includes a portion for reacting the successive samples with a reagent and a portion for analyzing the reaction product. It further includes introducing the reagent into the conduit upstream of the reaction portion to form a sample-reagent mixture, identifying the successive samples at the source, and responsive to such identification discontinuing the introduction of the reagent into the conduit and substituting therefore a pilot fluid displacing the mixture at the same flow rate along the reacting and analysis portions of the conduit.

Claims

1. A liquid sample analyzer, comprising:

means for flowing a stream of discrete respective aliquots of successive samples along first and second conduits from a sampler, said first and second conduits each including first means for reacting said aliquots of successive samples with a reagent and second means for viewing the reaction product, means for introducing a different reagent into each of said first and second conduits upstream of said first means thereof to form in each of said conduits a sample-reagent mixture, control means for identifying said successive samples at said sampler, and means responsive to said control means for discontinuing said introduction of said different reagent into either one of said first and second conduits with respect to selected ones of said samples, said control means being operative through said means responsive to said control means to introduce said pilot fluid into one of said first and second conduits while introducing said reagent into the other of said first and second conduits for flow in phased relation displacing said mixture along said first and second means.

3. Apparatus as defined in claim 1, wherein:

said means for introducing said pilot fluid comprises a third conduit having an inlet communicating with said one of said conduits into which said pilot fluid is introduced, said inlet being downstream from said viewing means, for recirculating said sample-reagent mixture through an outlet selectively in communication with the last-mentioned conduit upstream of said first means thereof.

2. A liquid sample analyzer, comprising:

means for flowing a stream of discrete samples successively along a first conduit from a sampler, said first conduit including first means for reacting said successive samples with a reagent and second means for viewing the reaction product, means for introducing a reagent into said first conduit upstream of said first means thereof to form in said conduit a sample-reagent mixture, control means for identifying said successive samples at said sampler, and means responsive to said control means for discontinuing said introduction of said reagent into said first conduit with respect to selected ones of said samples and for substituting therefor a pilot fluid in phased relation displacing said mixture at the same flow rate along said first conduit, said means for substituting of pilot fluid for said reagent comprising a second conduit having an inlet end communicating with said first conduit down stream from said viewing means thereof for recirculating said sample-reagent mixture through the outlet selectively in connection with said first conduit upstream of said first means thereof.

4. A method of analyzing liquid samples comprising the steps of:

flowing a stream of discrete aliquots of successive samples along respective first and second conduits from a source, each of which conduits includes a portion for reacting the successive samples with reagents and a portion for viewing the reaction product;

introducing a different reagent into said first and second conduits upstream of the reaction portion of each of the said conduits to form a sample-reagent mixture;

identifying the successive samples at the source; and

responsive to such identification discontinuing the introduction of said different reagent into either one of said first and second conduits and substituting therefor a pilot fluid for said reagent in one of said first and second conduits while introducing said reagent into the other of said first and second conduits for flow in phased relation at the same flow rate along the reacting and viewing portions.

6. A method as defined in claim 4, wherein:

said pilot fluid introduction includes recirculating said sample-reagent mixture passed through said viewing portion as a pilot fluid for reintroduction into said first and second conduits upstream of said sample flow thereinto.

5. A method of analyzing liquid samples comprising the steps of:

flowing a stream of discrete samples along a first conduit from a source, the conduit including a portion for reacting the successive samples with a reagent and a portion for viewing the reaction product;

introducing a reagent into said first conduit upstream of the reaction portion of said conduit to form a sample-reagent mixture;

identifying the successive samples at the source;

responsive to such identification discontinuing the introduction of said reagent into said first conduit and substituting therefor in phased relation a pilot fluid displacing the mixture at the same flow rate along the reacting and viewing portions of said first conduit, said pilot fluid introduction including recirculating the sample-reagent mixture passed through said analysis portion as said pilot fluid.