Analyzer for the determination of hemoglobin and other related compounds in whole blood and hemoglobin solutions
First Claim
1. An analyzer for the automatic testing of samples of 5 to 10 μ
- l of hemoglobin solutions, particularly whole blood, and for the automatic determination of the total hemoglobin and of the percentage contents of at least the deoxyhemoglobin, the carboxyhemoglobin and the oxyhemoglobin, and/or the ratio of the third to the first component, comprising a tonometer, in which a buffer is kept in equilibrium with a gas having a predetermined oxygen partial pressure, a first pump for the transfer of the liquid to be tested to a cuvette, an oxygen electrode, the sensitive part of which is contained within said cuvette in contact with the said liquid, a second pump for emptying the cuvette, a dual wavelength optical system for measuring the absorbances of the liquid present in the cuvette at the wavelengths of 497 and 565 nm, light sensors adapted to sense the light passing through said optical system, computer means adapted to be fed with electrical signals, respectively corresponding to the values sensed by the oxygen electrode and by the said light intensity sensors, and to process said signals according to the equations;
##EQU10## and display means adapted to display the results of the calculations carried out by said computer means.
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Abstract
This specification describes a new analyzer for the determination of total hemoglobin; percent oxyhemoglobin, deoxyhemoglobin, methmoglobin, sulfhemoglobin; and oxygen capacity in a micro sample (5 to 10 μl) of hole human blood. The essential components of the analyzer are (1) a cuvette in which simultaneous measurements of PO2 (by an oxygen electrode) and absorbance (by a dual wavelength optical system) are made, (2) an automatic pump system for filling and emptying the cuvette, (3) an equilibrating tonometer to equilibrate the reagent with air or another gas phase, (4) electronic circuits for the measurement of light intensity and oxygen pressure, (5) an analogue computer, and (6) a printer. Other essential parts of the invention are (a) original procedures and (b) ad hoc developed physico-chemical equations.
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Citations
10 Claims
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1. An analyzer for the automatic testing of samples of 5 to 10 μ
- l of hemoglobin solutions, particularly whole blood, and for the automatic determination of the total hemoglobin and of the percentage contents of at least the deoxyhemoglobin, the carboxyhemoglobin and the oxyhemoglobin, and/or the ratio of the third to the first component, comprising a tonometer, in which a buffer is kept in equilibrium with a gas having a predetermined oxygen partial pressure, a first pump for the transfer of the liquid to be tested to a cuvette, an oxygen electrode, the sensitive part of which is contained within said cuvette in contact with the said liquid, a second pump for emptying the cuvette, a dual wavelength optical system for measuring the absorbances of the liquid present in the cuvette at the wavelengths of 497 and 565 nm, light sensors adapted to sense the light passing through said optical system, computer means adapted to be fed with electrical signals, respectively corresponding to the values sensed by the oxygen electrode and by the said light intensity sensors, and to process said signals according to the equations;
##EQU10## and display means adapted to display the results of the calculations carried out by said computer means. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- l of hemoglobin solutions, particularly whole blood, and for the automatic determination of the total hemoglobin and of the percentage contents of at least the deoxyhemoglobin, the carboxyhemoglobin and the oxyhemoglobin, and/or the ratio of the third to the first component, comprising a tonometer, in which a buffer is kept in equilibrium with a gas having a predetermined oxygen partial pressure, a first pump for the transfer of the liquid to be tested to a cuvette, an oxygen electrode, the sensitive part of which is contained within said cuvette in contact with the said liquid, a second pump for emptying the cuvette, a dual wavelength optical system for measuring the absorbances of the liquid present in the cuvette at the wavelengths of 497 and 565 nm, light sensors adapted to sense the light passing through said optical system, computer means adapted to be fed with electrical signals, respectively corresponding to the values sensed by the oxygen electrode and by the said light intensity sensors, and to process said signals according to the equations;
Specification