Process for the preparation of insulin, analogs and derivatives thereof

• US 4,013,628 A
• Filed: 08/14/1975
• Issued: 03/22/1977
• Est. Priority Date: 08/16/1974
• Status: Expired due to Term

• Current Assignee: Hoechst Aktiengesellschaft (Sanofi )
• Original Assignee: Hoechst Aktiengesellschaft (Sanofi )
• Inventors: Geiger, Rolf, Obermeier, Rainer
• Primary Examiner(s): Gotts, Lewis
• Assistant Examiner(s): Suyat, Reginald J.
• Application Number:
  US05/604,667
• Time in Patent Office: 586 Days
• Field of Search: 260/112.7
• US Class Current: 530/303
• CPC Class Codes: C07K 14/62 Insulins
• CPC Class Codes: Y02P 20/55 Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Abstract

A process for the manufacture of insulin and derivatives thereof by treating an insulin derivative, wherein the A-chain is cross-linked with the B-chain by means of a sulfonyl diethyl-bisoxycarbonyl bridge and the B-chain may contain an acyl group in the 1-position, with alkali metal hydroxides or quaternary organic bases having a pH value of more than 13.

Claims

1. A method for making an insulin compound of the formula ##STR7## and biologically-active analogs thereof in which one or more amino acids have been exchanged for other, preferably simpler, amino acids or in which the chains are modified, preferably shortened, and in which Y is hydrogen or acyl, which method comprises treating a compound of the formula ##STR8## or an analog thereof as hereinbefore defined, with an alkali metal hydroxide or a quaternary organic base having a pH of more than 13.

2. An insulin compound of the formula ##STR9## and biologically-active analogs thereof in which one or more amino acids have been exchanged for other, preferably simpler, amino acids or in which the chains are modified, preferably shortened, and in which Y is hydrogen or acyl.