Enzyme electrode

• US 4,024,042 A
• Filed: 03/17/1976
• Issued: 05/17/1977
• Est. Priority Date: 03/27/1975
• Status: Expired due to Term

• Current Assignee: Servo Chem AB
• Original Assignee: Servo Chem AB
• Inventors: Enfors, Sven Olof, Molin, Nils Ludvig, Mosbach, Klaus Hermann, Nilsson, Hans Jorgen
• Primary Examiner(s): Tung, T.
• Application Number:
  US05/667,864
• Time in Patent Office: 426 Days
• Field of Search: 204/1 E, 204/195 P, 204/195 B, 195/103.5 R, 195/103.5 C, 195/121, 195/122
• US Class Current: 204/402
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The invention relates to an enzyme electrode which can be sterilized and regenerated and which has a sensitive surface and an enzyme in contact therewith. A semipermeable membrane is arranged at a distance from the sensitive surface of the detecting electrode and defines a chamber containing the enzyme which is supplied to and discharged from the chamber through a supply conduit and a discharge conduit, respectively. A sterilization device for the enzyme is arranged in the supply conduit for the enzyme. The enzyme electrode is produced by means of the detecting electrode being sterilized in a manner known per se and by means of the enzyme being led into a chamber via a sterilization device through a supply conduit for contact with the sensitive surface of the detecting electrode and for contact with the semipermeable membrane arranged at a distance from said sensitive surface. The enzyme is continuously or intermittently supplied through the supply conduit simultaneously as previously supplied enzyme is discharged from the chamber through the discharge conduit.

Claims

1. An enzyme electrode which can be sterilized and regenerated, comprising a detecting electrode having a sensitive surface and an enzyme in direct or indirect contact with the same, characterized in that a semi-permeable membrane is arranged at a distance from the sensitive surface of the detecting electrode and defines a chamber containing the enzyme and that a supply conduit and a discharge conduit are arranged for the supply and discharge of the enzyme, said supply conduit including a sterilization device for the enzyme.

2. Enzyme electrode according to claim 1, characterized in that the enzyme is present in the form of a solution.

3. Enzyme electrode according to claim 1, characterized in that the enzyme is present in the form of a suspension of immobilized enzymes.

4. Enzyme electrode according to claim 1, characterized in that the sterilization device is a membrane filter.

5. Enzyme electrode according to claim 1 characterized in that the sterilization device is a radiation sterilizer.