Superoxide dismutase and its application as an oxidation inhibitor

• US 4,029,819 A
• Filed: 08/22/1976
• Issued: 06/14/1977
• Est. Priority Date: 04/16/1973
• Status: Expired due to Term

• Current Assignee: Etablissement Public DIT Agence Nationale de Valorisation de La Recherche - Anvar
• Original Assignee: Etablissement Public DIT Agence Nationale de Valorisation de La Recherche - Anvar
• Inventors: Michelson, Adolf Michael
• Primary Examiner(s): Shapiro, Lionel M.
• Application Number:
  US05/713,670
• Time in Patent Office: 296 Days
• Field of Search: 195/126, 195/63, 426/61, 426/7
• US Class Current: 426/61
• CPC Class Codes: A23L 3/3463 Organic compounds; Microorganisms; Enzymes
• CPC Class Codes: A23L 3/3571 Microorganisms; Enzymes
• CPC Class Codes: C12N 9/0089 acting on superoxide as acceptor (1.15)

Abstract

The invention relates to a process for the production of superoxide dismutase and the superoxide dismutase so obtained. The superoxide dismutase obtained is extracted from marine bacterial strains and is characterized in that it comprises non-hematinic iron or copper and zinc, it has a molecular weight of about 33,000 to 40,000 ± 2500 and a pHi or isoelectric point of about 4 to 8.2 and it has maximum enzyme activity at a pH of about 8.5 to 10 with an optimum at about 9 to 9.5 and the use of said superoxide dismutases for the protection of oxidizable systems from auto-oxidation.

Claims

1. A composition protected from autooxidation comprising at least one member of the group consisting of lipids and antioxidants for foodstuffs and an effective amount of a superoxide dismutase of extracted from photobacterium marine bacterial cultures characterized by having a molecular weight of about 40,000 ±

2500, a pHi point of about 4 to 7, containing 1 to 2 atoms of non-hematinic iron per mole of superoxide dismutase and having a maximum enzymatic activity at a pH between 8.5 to 10.

2. The composition of claim 1 wherein the antioxidant is selected from the group consisting of pyrogallol and ascorbic acid.

3. A composition protected from autooxidation comprising at least one member of the group consisting of lipids and antioxidants for foodstuffs and an effective amount of a superoxide dismutase extracted from photobacterium marine bacterial cultures characterized by a molecular weight of 33,000 ±

2500, a pHi point of about 8.25, having a maximum enzymatic activity at a pH of 8.5 to 10 and containing about 1 atom of copper and about 2.0 atoms of zinc per mole of superoxide dismutase.

4. The composition of claim 18 wherein the antioxidant is selected from the group consisting of pyrogallol and ascorbic acid.

5. A method of protecting lipids and antioxidnts for foodstuffs from oxidation comprising incorporating into lipids and antioxidants for foodstuffs an effective amount of a superoxide dismutase of extracted from photobacterium marine bacterial cultures characterized by having a molecular weight of about 40,000 ±

2500, a pHi point of about 4 to 7, containing 1 to 2 atoms of non-hermatinic iron per mol of superoxide dismutase and having a maximum enzymatic activity at a pH between 8.5 to 10.

6. A method of protecting lipids and antioxidants for foodstuffs from oxidation comprising incorporating into lipids and antioxidants for foodstuffs an effective amount of a superoxide dismutase extracted from photobacterium marine bacterial cultures characterized by a molecular weight of 33,000 ±

2500, pHi point of about 8.25, having a maximum enzymatic activity at a pH of 8.5 to 10 and containing about 1 atom of copper and about 2.0 atoms of zinc per mole of superoxie dismutase.