Video fluorometer
First Claim
1. Apparatus for fluorescence spectroscopy comprising:
- a. means for holding a sample of fluorescent material;
b. means for simultaneously directing a spatially distributed spectrum of exciting energies onto said sample distributed along its length; and
c. means for simultaneously analyzing the set of fluorescence emissions given off by said sample along its length by producing a fluorescence intensity matrix of the excitation and emission spectra.
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Abstract
A fluoroescence spectrometer or fluorometer for simultaneously presenting the excitation and emission spectra of a multicomponent sample in the form of an intensity matrix is disclosed which comprises an elongated cuvette for holding the fluoroescent sample, along whose length a spatially distributed spectrum of exciting energies is simultaneously directed, and a polychromatic analyzer which simultaneously analyzes the excitation and emission spectra given off by the sample along its length by producing a spectrally dispersed image of the cuvette. The matrix of spectral intensities so obtained may be converted to electrical signals by a low-light-level television detector and displayed on a video monitor or processed with appropriate analog or digital computers for immediate analysis. The instrument can acquire 241 emission spectra taken at 241 different exciting wavelengths in 16.7 milliseconds.
33 Citations
19 Claims
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1. Apparatus for fluorescence spectroscopy comprising:
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a. means for holding a sample of fluorescent material; b. means for simultaneously directing a spatially distributed spectrum of exciting energies onto said sample distributed along its length; and c. means for simultaneously analyzing the set of fluorescence emissions given off by said sample along its length by producing a fluorescence intensity matrix of the excitation and emission spectra. - View Dependent Claims (2, 3, 4, 5)
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6. A fluorometer comprising:
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a. first polychromator means for producing a spatially distributed spectrum of exciting energies; b. a sample cuvette means for holding a sample of fluorescent material and having a long axis disposed such that said spatially distributed exciting energies from said first polychromator means excite said sample along its length; and c. second polychromator means, disposed adjacent to said sample cuvette means to receive the emitted energies given off by the excited sample along its length, for simultaneously analyzing the excitation and emission spectra of said sample by producing a fluorescence intensity matrix of the excitation and emission spectra. - View Dependent Claims (7, 8, 9, 10)
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11. A method of analyzing fluorescence spectra comprising the steps of:
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a. obtaining a sample of fluorescent material to be analyzed; b. directing a spatially distributed spectrum of exciting energies onto said sample distributed along an axis to excite said sample; and c. simultaneously analyzing the set of fluorescence emissions given off by said sample upon excitation along said axis by producing a fluorescence intensity matrix of the excitation and emission spectra. - View Dependent Claims (12, 13, 14, 15)
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16. A fluorescence spectrometer comprising:
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a. means having an axis for holding a sample of fluorescent material; b. means for directing exciting energy onto said sample distributed along the axis of said holding means; and c. means for simultaneously analyzing the set of fluorescence emissions given off by said sample along said axis over a predetermined range of emission wavelengths by producing a fluorescence intensity matrix of the excitation and emission spectra. - View Dependent Claims (17)
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18. A method of fluorescence analysis comprising the steps of:
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a. providing a sample of fluorescent material to be analyzed; b. directing exciting energy onto said sample distributed along an axis; and c. analyzing the emitted energies given off by the excited sample by simultaneously producing the excitation and emission spectra thereof in the form of a fluorescence intensity matrix. - View Dependent Claims (19)
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Specification