Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
First Claim
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1. The process of making an immunoradiometric quantitative assay of an unknown quantity of a known antigen of a first animal species comprising the steps of:
- a. preparing an antibody to the antigen of the first animal species in a second animal species,b. causing said antibody to be bound to a reaction site,c. reacting and binding said known antigen with said bound antibody,d. preparing an antibody to said known antigen in a third animal species and reacting and binding the same with the bound known antigen of paragraph c,e. preparing a purified, radioactive labeled antibody to the immunoglobulin of said third animal species in said second animal species and reacting and binding the same with the bound material of paragraph d,f. removing unreacted material from said reaction site, andg. measuring the radioactivity of the bound material to determine the quantity of the known antigen.
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Abstract
An improvement is made in 2-site immunoradiometric assay wherein a single labelled antibody can be used to assay many different antigens.
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3 Claims
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1. The process of making an immunoradiometric quantitative assay of an unknown quantity of a known antigen of a first animal species comprising the steps of:
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a. preparing an antibody to the antigen of the first animal species in a second animal species, b. causing said antibody to be bound to a reaction site, c. reacting and binding said known antigen with said bound antibody, d. preparing an antibody to said known antigen in a third animal species and reacting and binding the same with the bound known antigen of paragraph c, e. preparing a purified, radioactive labeled antibody to the immunoglobulin of said third animal species in said second animal species and reacting and binding the same with the bound material of paragraph d, f. removing unreacted material from said reaction site, and g. measuring the radioactivity of the bound material to determine the quantity of the known antigen. - View Dependent Claims (2, 3)
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Current AssigneeBoard of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
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Original AssigneeBoard of Trustees of the Leland Stanford Junior University (Stanford Management Co.)
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InventorsMiles, Laughton E. M.
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Primary Examiner(s)Padgett, Benjamin R.
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Assistant Examiner(s)Nucker, Christine M.
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Application NumberUS05/507,347Time in Patent Office1,020 DaysField of Search23/230 B, 424/1.5, 424/8, 424/12US Class Current436/518CPC Class CodesG01N 33/54306 Solid-phase reaction mechan...Y10S 436/804 Radioisotope, e.g. radioimm...Y10S 436/819 Multifunctional antigen or ...
