Treatment of cell culture microcarries

• US 4,036,693 A
• Filed: 02/02/1976
• Issued: 07/19/1977
• Est. Priority Date: 02/02/1976
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Levine, David W., Wang, Daniel I. C., Thilly, William G.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US05/654,134
• Time in Patent Office: 533 Days
• Field of Search: 195/1.8
• US Class Current: 435/403
• CPC Class Codes: C12M 25/16 Particles; Beads; Granular material; Encapsulation chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00
• CPC Class Codes: C12N 2533/70 Polysaccharides
• CPC Class Codes: C12N 5/0075 using microcarriers

Abstract

A method of treating certain cell culture microcarriers to improve their performance is disclosed. In this method, positively charged microcarriers, such as those produced by reacting polydextran beads with diethylaminoethyl, are treated by contacting them with macromolecular polyanions, such as carboxymethylcellulose, prior and/or during use in cultures. Such treatment overcomes deleterious effects previously observed in attempts to use these microcarriers in cell culture systems.

Claims

1. In the method of growing anchorage-dependent cells in a suspension of positively charged microcarriers in culture medium:

the improvement comprising treating said microcarriers by contacting them with a nontoxic macromolecular polyanion.

2. An improvement of claim 1 wherein said macromolecular polyanion is a cellulose.

3. An improvement of claim 2 wherein said macromolecular polyanion has a molecular weight of between about 600 and about 200,000.

4. An improvement of claim 3 wherein said microcarriers are positively-charged polydextran microcarriers.

5. An improvement of claim 1 wherein said macromolecular polyanion is carboxymethylcellulose.

6. An improvement of claim 4 wherein said macromolecular polyanion is carboxymethylcellulose.

7. An improvement of claim 6 wherein said microcarriers are contacted with said carboxymethylcellulose prior to cell inoculation.

8. An improvement of claim 7 wherein medium replenishments containing carboxymethylcellulose are added to said culture medium.

9. A method of treating positively-charged cell culture microcarriers to enhance their cell growing characteristics comprising contacting said microcarriers with a nontoxic macromolecular polyanion.

10. A method of claim 9 wherein said macromolecular polyanion is a negatively-charged polysaccharide or protein.

11. A method of claim 9 wherein said macromolecular polyanion is carboxymethylcellulose.

12. A method of claim 11 wherein said microcarriers are positively-charged polydextran microcarriers.

13. Cell culture microcarriers produced by the method of claim 9.

14. Cell culture microcarriers produced by the method of claim 12.