Anaerobic environmental system for bacteria culture testing
First Claim
1. A package for storing and transporting an anaerobic culture comprising:
- a bag of flexible sheet material of low gas permeability;
a self-contained gas generating apparatus in the bag for generating at least a reducing gas;
a culture retaining receptacle in the bag; and
a catalyst in the bag which promotes reaction between the reducing gas, when produced by the gas generating apparatus, and oxygen in the bag;
said gas generating apparatus comprising a container having an opening, a reducing gas generating solid material in the container, an ampoule containing a liquid which is reactive with the solid material to produce a reducing gas catalytically reactive with oxygen at room temperature, said ampoule being openable from outside the container to free the liquid to contact the solid material, and means in the container which prevents liquid from flowing from the container after the ampoule is opened but which permits flow of reducing gas generated in the container out of the opening.
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Accused Products
Abstract
A package for storing, incubating or transporting an anaerobic culture comprising a bag of flexible sheet material of low gas permeability, a self-contained gas generating apparatus in the bag for generating at least a reducing gas, a culture retaining container in the bag, and a catalyst in the bag which promotes reaction between the reducing gas, when produced by the gas generating apparatus, and oxygen in the bag. The bag may also contain a color indicator apparatus which when activated indicates the presence or absence of oxygen in the bag by color change. Hydrogen is the preferred reducing gas which is generated, alone or in conjunction with carbon dioxide.
34 Citations
35 Claims
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1. A package for storing and transporting an anaerobic culture comprising:
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a bag of flexible sheet material of low gas permeability; a self-contained gas generating apparatus in the bag for generating at least a reducing gas; a culture retaining receptacle in the bag; and a catalyst in the bag which promotes reaction between the reducing gas, when produced by the gas generating apparatus, and oxygen in the bag; said gas generating apparatus comprising a container having an opening, a reducing gas generating solid material in the container, an ampoule containing a liquid which is reactive with the solid material to produce a reducing gas catalytically reactive with oxygen at room temperature, said ampoule being openable from outside the container to free the liquid to contact the solid material, and means in the container which prevents liquid from flowing from the container after the ampoule is opened but which permits flow of reducing gas generated in the container out of the opening. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A package for storing and transporting an anaerobic culture comprising:
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a bag of flexible sheet material of low gas permeability; a self-contained gas generating apparatus in the bag for generating at least a reducing gas; a culture retaining receptacle in the bag; a catalyst in the bag which promotes reaction between the reducing gas, when produced by the gas generating apparatus, and oxygen in the bag; and a color indicator apparatus in the bag which when activated indicates the presence or absence of oxygen in the bag by color change; said color indicator comprising a container which permits flow of gas thereto, an ampoule in the container, said ampoule containing a redox color indicator liquid, and an absorbent material, for the liquid in the ampoule, in the container. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30)
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31. A package for use in storing and transporting an anaerobic culture comprising:
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a bag of flexible sheet material of low gas permeability; a self-contained gas generating apparatus in the bag for generating at least a reducing gas; a catalyst inside of the bag which promotes reaction between the reducing gas, when produced by the gas generator apparatus, and oxygen in the bag; a color indicator apparatus which when activated indicates the presence or absence of oxygen in the bag by color change; and the gas generating apparatus comprising; a container having an opening, a reducing gas generating solid material in the container, an ampoule containing a liquid which is reactive with the solid material to produce a reducing gas catalytically reactive with oxygen at room temperature, said ampoule being openable from outside the container to free the liquid to contact the solid material, and means in the container which prevents liquid from flowing from the container after the ampoule is opened but which permits flow of reducing gas generated in the container out of the opening. - View Dependent Claims (33, 34, 35)
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32. A package for use in storing and transporting an anaerobic culture comprising:
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a bag of flexible sheet material of low gas permeability; a self-contained gas generating apparatus in the bag for generating at least a reducing gas; a catalyst inside of the bag which promotes reaction between the reducing gas, when produced by the gas generator apparatus, and oxygen in the bag; a color indicator apparatus which when activated indicates the presence or absence of oxygen in the bag by color change; and the color indicator comprising; an open container which permits flow of gas therethrough, an ampoule in the container, said ampoule containing a redox color indicator liquid, and an absorbent material, for the liquid in the ampoule, in the container.
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Specification