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Polarographic analysis of cholesterol and other macromolecular substances

  • US 4,040,908 A
  • Filed: 03/12/1976
  • Issued: 08/09/1977
  • Est. Priority Date: 03/12/1976
  • Status: Expired due to Term
First Claim
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1. A method for quantitative polarographic determination of a substance which is convertible by at least one enzyme to produce ultimately hydrogen peroxide comprising the steps ofproviding a polarographic cell including at least one electrode sensitive to hydrogen peroxide positioned behind a membrane permeable to hydrogen peroxide and impermeable to said substance being measured, said cell containing an electrolyte,at least one enzyme contained in said cell on the side of the membrane opposite said electrode for enzymatic reaction with said substance to produce ultimately hydrogen peroxide,establishing a potential across said cell such that current is produced which is proportional to the amount of hydrogen peroxide produced,adding a quantity of material containing said substance for said enzymatic reaction on the side of the membrane opposite said electrode and to effect diffusion of at least a portion of said hydrogen peroxide into said membrane and into contact with said electrode, anddetermining the current flowing across said cell as a function of the amount of hydrogen peroxide formed and as an indication of the amount of said substance in said material.

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