Integral element for analysis of liquids
First Claim
1. An integral element for analysis of liquids, the element comprising,a reagent layer comprising a composition that is interactive in the presence of a predetermined analyte to provide a diffusible, detectable species,a non-fibrous radiation-blocking layer, permeable to the detectable species and comprising an opacifying agent,a radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a non-fibrous material,wherein the radiation-blocking layer is interposed between the registration layer and the reagent layer.
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Abstract
The present invention concerns a multilayer element for the analysis of liquids such as biochemical and biological liquids. Elements according to the invention include (1) a reagent layer including a composition that is interactive in the presence of a predetermined substance to be analyzed (analyte) to provide a diffusible, detectable species, and (2) a registration layer that is permeable to the detectable species and within which such species, e.g., a dye, can be detected. Also within the element, preferably between the reagent layer and the registration layer, there can be a radiation-blocking layer, such as an opaque reflecting layer, to enhance detection of the diffusible species within the registration layer. The element can also include a spreading layer, preferably separated from the registration layer by a reagent layer. In operation, a sample of liquid under analysis is applied to the reagent layer of the element or, if present, to a spreading layer. If the sample contains analyte that the element is intended to detect, chemical reaction or other interaction within the reagent layer provides a detectable species that diffuses, via any intervening layers such as a radiation-blocking layer, into the registration layer for detection there, such as by radiometric techniques like reflection spectrophotometry.
247 Citations
20 Claims
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1. An integral element for analysis of liquids, the element comprising,
a reagent layer comprising a composition that is interactive in the presence of a predetermined analyte to provide a diffusible, detectable species, a non-fibrous radiation-blocking layer, permeable to the detectable species and comprising an opacifying agent, a radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a non-fibrous material, wherein the radiation-blocking layer is interposed between the registration layer and the reagent layer.
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2. An integral element for analysis of liquids, the element comprising a radiation-transmissive support having thereon,
a reagent layer comprising a composition that is interactive in the presence of a predetermined analyte to provide a diffusible, detectable species, a non-fibrous radiation-blocking layer, permeable to the detectable species and comprising an opacifying agent, and a radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a non-fibrous material, wherein the registration layer is interposed between the support and the radiation-blocking layer and the radiation-blocking layer is interposed between the registration layer and the reagent layer.
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6. An integral element for analysis of liquids, the element comprising,
an isotropically porous spreading layer comprising a non-fibrous material, a reagent layer comprising a composition that is interactive in the presence of a predetermined analyte to provide a diffusible, detectable species, and a radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a non-fibrous material wherein the reagent layer is interposed between the registration layer and the spreading layer.
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7. An integral element for analysis of liquids, the element comprising a radiation-transmissive support having thereon,
an isotropically porous spreading layer comprising a non-fibrous material, a reagent layer comprising a composition comprising material interactive in the presence of a predetermined analyte to provide a diffusible, detectable species, and a radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a non-fibrous material, wherein the registration layer is interposed between the support and the reagent layer and the reagent layer is interposed between the registration layer and the spreading layer.
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9. An integral element for analysis of liquids the element comprising a radiation-transmissive support having thereon,
a water-resistant, isotropically porous spreading layer comprising a non-fibrous material, a water-swellable reagent layer comprising a composition that is interactive in the presence of a predetermined analyte to provide a diffusible, detectable species, a non-fibrous, radiation-blocking layer, permeable to the detectable species and comprising an opacifying agent, and a radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a water-swellable, non-fibrous material, wherein the registration layer is the closest to the support of said layers the radiation-blocking layer is interposed between the reagent layer and the registration layer, and the reagent layer is interposed between the radiation-blocking layer and the spreading layer.
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10. An integral element for analysis of liquids, the element comprising a radiation-transmissive support having thereon,
a water-resistant, isotropically porous spreading layer comprising a non-fibrous polymeric composition or particulate matter, a water-swellable reagent layer comprising a composition that is interactive in the presence of a predetermined analyte to provide a diffusible detectable species, a non-fibrous radiation-blocking layer, permeable to the detectable species and comprising an opacifying agent, and a water-swellable, non-fibrous radiation-transmissive registration layer, permeable to the detectable species and within which said species can be detected, wherein the registration layer is the closest to the support of said layers, the radiation-blocking layer is interposed between the reagent layer and the registration layer, and the reagent layer is interposed between the radiation-blocking layer and the spreading layer.
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15. An integral element for analysis of liquids, the element comprisng a radiation-transmissive support having thereon,
a water-resistant, isotropically porous spreading layer comprising a blushed polymer and a surfactant, a reagent layer comprising a water-swellable hydrophilic colloid having distributed therein a composition that is interactive in the presence of a predetermined analyte to provide a diffusible, detectable species, a radiation-blocking layer permeable to the detectable species and comprising a hydrophilic colloid having a pigment distributed therein, and a water-swellable, radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a hydrophilic colloid, wherein the registration layer is the closest to the support of said layers the radiation-blocking layer is interposed between the reagent layer and the registration layer, and the reagent layer is interposed between the radiation-blocking layer and the spreading layer.
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18. An integral element for analysis of liquids, the element comprising a radition-transmissive support having thereon,
a water-resistant, isotropically porous spreading layer comprising a blushed polymer selected from cellulose acetate and a polyamide, an inorganic pigment and a surfactant, a water-swellable reagent layer comprising a hydrophilic colloid selected from gelatin, poly(vinyl alcohol), poly(vinyl pyrrolidone), an acrylamide, agarose and a polysaccharide, said colloid having distributed therein a composition that is interactive in the presence of a predetermined analyte to provide a diffusible detectable species, a radiation-blocking layer, permeable to the detectable species and comprising a hydrophilic colloid selected from gelatin, poly(vinyl alcohol), poly(vinyl pyrrolidone), an acrylamide, agarose and a polysaccharide, said colloid having distributed therein a pigment selected from carbon, titanium dioxide and barium sulfate and a water-swellable, radiation-transmissive registration layer, permeable to the detectable species, within which said species can be detected and comprising a hydrophilic colloid selected from gelatin, poly(vinyl alcohol), poly(vinyl pyrrolidone), an acrylamide, agarose and a polysaccharide, and a mordant for the detectable species, wherein the registration layer is the closest to the support of said layers, the radiation-blocking layer is interposed between the reagent layer and the registration layer, and the reagent layer is interposed between the radiation-blocking layer and the spreading layer.
Specification