Light measuring apparatus

• US 4,055,768 A
• Filed: 09/07/1976
• Issued: 10/25/1977
• Est. Priority Date: 09/07/1976
• Status: Expired due to Term

• Current Assignee: Nathan S. Bromberg
• Original Assignee: Nathan S. Bromberg
• Inventors: Bromberg, Nathan S.
• Primary Examiner(s): Willis, Davis L.
• Application Number:
  US05/721,065
• Time in Patent Office: 413 Days
• Field of Search: 250/364, 250/458, 250/461 R, 250/461 B, 356/85
• US Class Current: 250/461.2
• CPC Class Codes: G01N 2021/1738 Optionally different kinds of measurements; Method being valid for different kinds of measurement
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6486 Measuring fluorescence of biological material, e.g. DNA, RNA, cells G01N21/6428 takes precedence
• CPC Class Codes: G01N 2201/0696 Pulsed

Abstract

Apparatus usable as a fluorometer for measuring the concentration of fluorescent material in a specimen employs a flashing lamp to illuminate the specimen with excitation radiation. The intensity of the fluorescence emitted by the specimen is therefore modulated at the flash rate. That modulated fluorescence is converted by a photodetector to an electrical signal. The electrical signal is amplified and is passed through a phase sensitive detector to an integrator. The phase sensitive detector is regulated to operate at a rate related to the flash rate whereby an appropriately rectified signal is fed to the integrator. When the specimen has been subjected to a selected amount of excitation radiation, a signal is generated which marks the end of the integration interval. The accumulated signal in the integrator is then measured to obtain a measurement of the quantity of fluorescent material in the specimen. The apparatus is also usable as a nephelometer to measure the light scattering properties of a specimen illuminated by the flashing lamp.

Claims

1. Apparatus of the type for measuring light emitted, scattered, or reflected from a specimen, the apparatus comprising

a. a source of excitation radiation arranged to illuminate the specimen,

b. modulating means for modulating the excitation radiation,

c. means for emitting a K signal when the specimen has been subjected to a selected amount of excitation radiation,

d. a photodetector arranged to provide an electrical signal in response to the intensity of the light emitted, scattered, or reflected by the specimen,

d. means for amplifying the output signal of the photodetector,

f. a phase sensitive detector having the amplified signal applied to its input,

g. means coupled to the modulating means and deriving signals therefrom which regulate the operation of the phase sensitive detector,

h. an integrator having its input coupled to the output of the phase sensitive detector, and

i. means responsive to the K signal for measuring the quantum of the signal accumulated in the integrator at the emission of the K signal.

2. Apparatus according to claim 1 wherein

A. the source of excitation radiation is arranged to be intermittently energized by a.c. electrical power to produce excitation radiation, and

B. the modulating means is arranged to cause half cycles of the a.c. electrical power which are alternately of different polarity to energize the excitation radiation source and the interval between successive half cycles is at least two half cycles in duration.

3. Apparatus according to claim 1, wherein the means responsive to the K signal includesa source of constant current,a switch for connecting the constant current source to the input of the integrator in response to the reception of the K signal,a comparator connected to the output of the integrator for emitting a signal when the output of the integrator reaches a zero reference level, andclock means for measuring the interval of time required for the constant current source to cause the output of the integrator to reach the zero reference level.

4. Apparatus according to claim 3 further including means for setting the apparatus to a zero datum comprising

a. a source of electrical potential for supplying the zero reference level to an input of the comparator,

b. a modulator for modulating the zero reference level signal applied to the input of the comparator, and

c. means coupled to the output of the comparator for indicating activation of the comparator at the modulation rate, and for indicating settings above or below the modulated zero reference level.

5. Apparatus according to claim 1 wherein the means for emitting a K signal when the specimen has been subjected to a selected amount of excitation radiation includesa second photodetector arranged to supply an electrical signal in response to the intensity of the excitation radiation emitted by the sourcea second phase sensitive detector deriving its input from the output of the second photodetectora second integrator having its input fed by the output of the phase sensitive detector, anda second comparator coupled to the output of the second integrator, the second comparator emitting the K signal upon the output of the second integrator attaining a reference level.

6. Apparatus according to claim 5 wherein

the second phase sensitive detector is regulated by the same control signals that regulate the operation of the first mentioned phase sensitive detector.