Apparatus for counting and/or measuring particles suspended in a fluid medium

• US 4,056,324 A
• Filed: 05/05/1976
• Issued: 11/01/1977
• Est. Priority Date: 05/10/1975
• Status: Expired due to Term

• Current Assignee: Hildegard Gohde
• Original Assignee: Hildegard Gohde
• Inventors: Gohde, Hildegard
• Primary Examiner(s): Corbin, John K.
• Assistant Examiner(s): Koren, Matthew W.
• Application Number:
  US05/683,340
• Time in Patent Office: 545 Days
• Field of Search: 356/181, 356/246, 250/576
• US Class Current: 356/246
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing

Abstract

An apparatus for counting and/or measuring small particles suspended in a fluid medium, is provided with an improved flow cell in which the particle carrying fluid medium is fed into a flow channel through which also a particle free liquid is passed for narrowing and extending the flow of the suspension fluid within the channel which by its configuration including a section of continuously decreasing cross sectional area, allows a more precise measurement and avoids clogging of the suspension fluid inlet the diameter of which is not any longer a critical dimension.

Claims

1. In an apparatus for counting and/or measuring particles suspended in a fluid medium,

a flow cell having spaced apart cover wall means and bottom wall means for defining a flow channel therebetween,

a first inlet to said channel and an outlet from said channel at opposite ends thereof for establishing a general direction of flow in said channel going from said first inlet to said outlet,

a second inlet to said channel in said bottom means between and spaced from said first inlet and said outlet,

means in said cover wall means bordering a section of said channel between said second inlet and said outlet defining a window extending substantially parallel with said general direction of flow within said section,

said bottom wall means including a substantially plane surface opposite said window and inclined relative to said general direction of flow so that the cross-sectional area of said channel is reduced in said general direction, and

observation means having an axis of observation passing through said window for defining an observation area between said window and adjacent said inclined plane surface, said observation area being a plane extending from said inclined plane surface into said channel parallel to said window.

2. A flow cell for the examination of biological cells or small particles suspended in a fluid medium, comprising

bottom wall means, sidewall means and cover means defining a substantially straight flow channel,

a first inlet and an outlet at opposite ends of said channel,

a second inlet to said channel in said bottom wall means between and spaced apart from said first inlet and said outlet,

the section of said channel between said second inlet and said outlet being bounded by a plane transparent part of said cover means, two parallel plane parts of said sidewall means, and a plane part of said bottom wall means,

said parts defining a channel section having a rectangular cross section,

said plane part of said bottom wall means being inclined relative to said plane transparent part so that said rectangular cross section is reduced in the direction toward said outlet.

3. An apparatus for counting and/or measuring small particles suspended in a fluid medium, comprising

a source of particle-free liquid,

a source of particle-containing suspension liquid,

a radiation source,

means for detecting and recording radiation, and

a flow cell,

said flow cell having

a bottom wall,

sidewalls and

a cover defining a flow channel having a first inlet and an outlet at opposite ends thereof,

a second inlet in said bottom between and spaced apart from said first inlet and said outlet,

means for connecting said first inlet with said source of particle-free liquid,

means for connecting said second inlet to said source of suspension liquid, said channel comprising

means defining a channel portion between said second inlet and said outlet having a cross-sectional area which gradually decreases in the direction toward said outlet,

said section being bordered by a plane transparent window part of said cover and by a part of said bottom inclined relative to said window part,

said radiation means and said detecting and recording means having a common axis normal to said window part,

said detecting means including optical means defining a detecting area on said axis within said channel in a plane parallel to said window part and contiguous to said inclined portion of said bottom.

4. An apparatus according to claim 3 wherein said inclined portion of said bottom has a plane surface.

5. Apparatus according to claim 3 wherein said sidewalls of said channel are substantially planar and parallel to each other.

6. Apparatus according to claim 3 wherein said second inlet is located in a part of said channel having constant cross sectional area and in which said section of gradually decreasing cross sectional area is spaced apart from said second inlet.

7. Apparatus according to claim 3 wherein said channel portion of gradually decreasing cross sectional area is connected to the outlet by a part of said channel having constant cross sectional area.

8. Apparatus according to claim 3 wherein said inclined portion of said bottom is transparent.