Stable blood plasma, process for preparing it and its use as comparative plasma in coagulation tests

• US 4,056,484 A
• Filed: 12/29/1975
• Issued: 11/01/1977
• Est. Priority Date: 12/31/1974
• Status: Expired due to Term

• Current Assignee: Behringwerke Aktiengesellschaft (Sanofi )
• Original Assignee: Behringwerke Aktiengesellschaft (Sanofi )
• Inventors: Heimburger, Norbert, Schwinn, Horst, Sieber, Axel
• Primary Examiner(s): Padgett, Benjamin R.
• Assistant Examiner(s): Gluck, Irwin
• Application Number:
  US05/644,519
• Time in Patent Office: 673 Days
• Field of Search: 252/408, 252/299, 62/60, 23/230 B, 424/2, 424/11, 424/101, 195/103.5 C
• US Class Current: 436/16
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0221 Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
• CPC Class Codes: A61K 35/16 Blood plasma; Blood serum umbilical cord blood A61K35/51
• CPC Class Codes: F26B 5/06 the process involving freezing
• CPC Class Codes: G01N 2496/05 containing blood cells or plasma
• CPC Class Codes: G01N 33/96 involving blood or serum control standard
• CPC Class Codes: Y10T 436/106664 Blood serum or blood plasma standard or control
• CPC Class Codes: Y10T 436/107497 Preparation composition [e.g., lysing or precipitation, etc.]
• CPC Class Codes: Y10T 436/108331 Preservative, buffer, anticoagulant or diluent

Abstract

Lyophilized blood plasma under a protective gas wherein the protective gas contains at least 5% of carbon dioxide.

Claims

1. Lyophilized blood plasma containing an anti-coagulating agent and present in a sealed container under a protective gas containing at least 5 percent of carbon dioxide.

2. Lyophilized blood plasma as in claim 1 which additionally contains a colloid-stabilizing additive.

3. A method for making lyophilized blood plasma as in claim 1, which method comprises mixing fresh blood with an anticoagulating agent, centrifuging said mixture and separating the plasma component thereof, filling said plasma into a non-wettable container, freezing said plasma to form frozen plasma having a large surface area, lyophilizing said frozen plasma, evacuating said container, introducing said protective gas into said container, and then sealing said container.

4. In a method for determining a component of blood plasma by observing the coagulation behavior of a plasma sample to be tested and comparing this behavior with the coagulation behavior of a plasma of known characteristics, the improvement wherein said plasma of known characteristics is prepared from lyophilized blood plasma as in claim 1.