Calibration in a blood analyzer

• US 4,061,469 A
• Filed: 12/08/1975
• Issued: 12/06/1977
• Est. Priority Date: 12/06/1974
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim Diagnostics Corp. (Roche Holding AG)
• Original Assignee: Boehringer Mannheim Diagnostics Corp. (Roche Holding AG)
• Inventors: DuBose, Charles Ray
• Primary Examiner(s): Serwin, R.E.
• Application Number:
  US05/638,472
• Time in Patent Office: 729 Days
• Field of Search: 23/253 R, 23/230 R, 23/259 R, 23/230 B, 356/39, 356/36, 235/151.35
• US Class Current: 422/64
• CPC Class Codes: G01N 35/00594 Quality control, including calibration or testing of components of the analyser
• CPC Class Codes: G01N 35/00693 Calibration

Abstract

In a blood analyzer, improved calibration is provided. First and second photodetectors sense energy supplied directly from a source and from the source through a sample respectively. A log ratio amplifier provides an analog output indicative to the log of the ratio of the outputs of the photodetectors to a control circuit. When a blank sample is measured, a programmable voltage source operates to set the analog output to a selected level. A reference sample having optical density corresponding to a preset value is next measured, and a variable gain amplifier is set by the control circuit to cause the analog signal to correspond to the preset value.

Claims

1. In a spectrophotometric measuring system for analyzing the concentration of a substance in a sample as a function of optical density of the sample, calibration means comprising first and second photodetectors positioned for respectively receiving radiant energy of a source and radiant energy applied from the source through a sample;

ratio means connected to said photodetectors for providing an output as a function of the ratio of the outputs of said first and second detectors;

a programmable voltage source;

summing amplifier means having outputs from said ratio circuit and from said programmable voltage source coupled thereto for summing the outputs thereof, measuring means for measuring an output indicative of the output of said summing amplifier;

a progammable gain amplifier coupled between said programmable voltage source and said measuring means;

control means connected to said measuring means, and providing outputs coupled to program said programmable voltage source and said programmable gain amplifier;

blank mode selection means for selection in correspondence with placing of a blank sample between said source and said second photodetector for connecting said control means for setting gain of said programmable gain amplifier at a preselected level and for providing a programming signal to said programmable voltage source;

said control means further comprising blank level means for providing a value indicative of a desired blank level;

means for comparing the output of said summing means to the output of said blank level means and for providing the programming signal to reduce the error between the value and the output of said summing means below a predetermined level, and means for holding and applying said programming signal to said programmable voltage source in an operate mode in which an unknown sample is placed between the source and said second photodetector.

2. Apparatus according to claim 1 further comprising reference mode selection means for selection in correspondence with placing a reference sample between said source and said second photoconductor for connecting said control means for providing the programming signal to said programmable voltage source and for determining a gain control signal for said programmable gain amplifier;

said control means further comprising storage means for storing a preset value indicative of a desired signal to be obtained from said measuring means in response to placing of the reference sample between said source and said second photodetector, and wherein said means for comparing further comprises means for comparing the output of said measuring means to said preset value and for providing the gain control signal to reduce the difference between the output of said measuring means and the desired signal below a predetermined level, and means for holding and applying said gain control signal to said programmable gain amplifier in an operate mode, whereby resolution of said measuring system is improved.

3. Apparatus according to claim 2 wherein said ratio circuit comprises a log ratio circuit.

4. Apparatus according to claim 3 wherein said blank level means comprises means for providing a value indicative of analog ground.

5. Apparatus according to claim 4 comprising a plurality of first and second photodetectors, each pair of first and second photodetector being associated with one channel, first multiplexing means for multiplexing channel outputs to said ratio circuit, second multiplexing means synchronized with said first multiplexing means for operating said control circuit for individually processing outputs of said measuring means for producing the programming signal and gain control signal for each channel when operating in the blank mode and reference mode respectively.

6. Apparatus according to claim 5 wherein said control means further comprises means for storing each programming and gain control signal for each channel.

7. Apparatus according to claim 6 wherein said control means further comprises accessing and multiplexing means synchronized with said first multiplexing means for accessing during an operate mode programming and gain control signals corresponding to the channel for which outputs are provided from the first and second photodetectors to said ratio circuit and coupling the programming and gain control signals to said programmable voltage source and programmable gain amplifier respectively;

and further comprising means for clearing said programming and gain control signals to enable application of programming and gain control signals corresponding to another channel to said programmable voltage source and said programmable gain amplifier.

8. Apparatus according to claim 7 wherein said measuring means comprises an analog-to-digital converter for supplying output signals to a digital register.

9. Apparatus according to claim 8 wherein said programmable voltage source comprises a digital-to-analog converter and said programming signal comprises a digital word having respective bits coupled to successive stages of said digital-to-analog converter.

10. Apparatus according to claim 9 wherein said programmable gain amplifier comprises a digital-to-analog converter coupled to an operational amplifier for determining the gain thereof and said gain control signal is a digital word having respective bits coupled to successive stages of said digital-to-analog converter.