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Detection of catalase-containing bacteria

  • US 4,065,357 A
  • Filed: 11/17/1975
  • Issued: 12/27/1977
  • Est. Priority Date: 01/21/1974
  • Status: Expired due to Term
First Claim
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1. A process for the clinical detection of the catalase activity in catalasa-containing cells in body fluid by dynamic monitoring of oxygen gas produced by the catalase-peroxide reaction comprising the steps of:

  • a. secruing a quantity of body fluid of unknown catalase content,b. intorducing a predetermined volume of said body fluid into a non-expandable reaction volume separated by an oxygen permeable membrane from electrochemical measuring means, said electrochemical measuring means being in association with said oxygen permeable membrane said means being totally outside said closed reaction volume, said membrane being impermeable to hydrogen peroxide and having an oxygen permeability coefficient of at least about 5 ×

    10-11 gms./atmos.-cm.-sec.,c. introducing a predetermined quantity of hydrogen peroxide solution into said reaction volume such that the volumes of said body fluid and hydrogen peroxide solution completely fill said reaction volume and such that the resulting solution has a hydrogen peroxide concentration in the range of from 0.01 to 1 mole per liter of said resulting solution, closing said reaction volume andd. electrochemically measuring the increase of partial pressure of oxygen in said reaction volume by measuring the extent of diffusion of oxygen through said membrane, said extent of diffusion of oxygen through said membrane being linearly proportional to said partial pressure of oxygen in said reaction volume.

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