Method and apparatus for determining hematocrit

• US 4,068,169 A
• Filed: 09/21/1976
• Issued: 01/10/1978
• Est. Priority Date: 09/21/1976
• Status: Expired due to Term

• Current Assignee: The First National Bank of Boston (Bank of America Corp.)
• Original Assignee: Boehringer Mannheim Diagnostics Corp. (Roche Holding AG)
• Inventors: Angel, Henry Robert, Bachenheimer, Bernard Otto
• Primary Examiner(s): Rolinec, Rudolph V.
• Assistant Examiner(s): Sunderdick, Vincent J.
• Application Number:
  US05/725,268
• Time in Patent Office: 476 Days
• Field of Search: 324/71 CP, 235/92 PC, 328/111, 328/115
• US Class Current: 324/71.1
• CPC Class Codes: G01N 15/132 Circuits
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

An apparatus and method are provided for measuring hematocrit. A diluted blood sample is passed through a well-known conductivity sensor providing a sensor pulse for each sensed blood cell. Each pulse has a peak amplitude corresponding to the size of a blood cell passing through the sensor. During a first time period initiated by passing of the sensor pulse above a threshold level, the sensor pulse is coupled to peak detecting means, and a signal indicative of the level of the peak is generated and stored. The value of each stored signal is added to an integrator for a fixed period of time. The value stored by the integrator is thus indicative of hematocrit level for a known sample volume.

Claims

1. Apparatus for measuring hematocrit comprising:

peak detection means for connection to a sensor providing sensor pulses in response to blood cells in a sample, the sensor pulses each having a peak amplitude respectively corresponding to the size of a cell in response to which the sensor pulse is produced;

storage means connected to said peak detecting means for generating and storing a signal having a level indicative of the peak value sensed by said peak detecting means for each pulse;

accumulation means;

controlled switching means coupling said storage means to said accumulation means, timing means responsive to said sensor pulses for initiating a first time period in response to each sensor pulse in excess of a threshold level and providing an output for enabling said storage means to respond to the sensor pulse, said timing means further including means for establishing a second time period in response to the completion of each first time period and providing an output for closing said controlled switching means for a fixed time period during said second time period, whereby said accumulation means is incremented by an amount in correspondence with the level of the stored signal in said storage means.

2. Hematocrit measuring apparatus according to claim 1, wherein said timing means further comprises means for establishing each said second time period at the end of each said first time period.

3. Hematocrit measuring apparatus according to claim 2 wherein said peak detector comprises a high impedance amplifier and said storage means is connected in a feedback amplifier, said timing means further comprises means for providing a reset signal to said storage means at the end of a second time period, said storage means comprises an analog integrator, said accumulation means comprises an analog integrator, and said means for coupling said storage means to said accumulation means comprises means for closing said controlled switching means for a fixed period of time during said second time period.

4. Hematocrit measuring apparatus according to claim 3 further comprising means for removing said resetting signal from said storage means in response to initiation of a next first time period following a first second time period.

8. Hematocrit measuring apparatus according to claim 4 further comprising controlled switching means connected across said accumulation means and timing means for selectively maintaining said controlled switching means in an open state whereby accumulation means is enabled to accumulate a sum of the integrals of a plurality of stored signals from said storage means.

9. Hematocrit measuring apparatus according to claim 8 further comprising compensating input means for storing drift signals produced in said accumulation means and connected for common control by said timing means connected to said controlled switching means across said accumulation means.

5. Apparatus according to cliam 4 wherein said means establishing first time period comprises a threshold amplifier coupled to the sensor providing said sensor pulses, a one shot multivibrator connected for triggering by said threshold amplifier, and gating means connected to said threshold amplifier and said one shot multivibrator for providing a timing signal having a duration equal to the longer of the output of said one shot multivibrator or said threshold amplifier.

6. Hematocrit measuring apparatus according to claim 5 wherein said means for establishing said second time period comprises a second one shot multivibrator having said timing signal connected thereto for initiating said second time period on the expiration of said first time period.

7. Hematocrit measuring apparatus according to claim 6 wherein said controlled switching means comprises an analog switch having a control terminal coupled to the output of said second one shot multivibrator.

10. Hematocrit measuring apparatus according to claim 7 further comprising scaling means connected to the output of said accumulation means for relating the output level of said accumulation means to a total volume of a sample whereby the output of said scaling means may be normalized in units of hematocrit percentage.

11. A method of measuring hematocrit comprising the steps of detecting the peak of each sensor pulse indicating a size of a blood cell in a sample passing through a sensor, establishing a first time period equal to at least the width of a sensor pulse, generating a signal indicative of the height of the sensor pulse within the first time period and storing the signal to provide a stored signal, establishing a second time period in response to the end of said first time period and incrementing accumulation means for a fixed time period by an amount in correspondence with said stored signal.

12. A method according to claim 11 wherein the step of establishing said first time period comprises establishing a said first time period equal to the longer of the width of the sensor pulse or a preselected time period.

13. A method according to claim 12 wherein the step of establishing said second time period comprises initiating said second time period at the end of said first time period.

14. The method according to claim 13 further comprising the step of normalizing the indication in said accumulation means to provide an output in units of hematocrit percentage.