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Colorimetric assay for bilirubin

  • US 4,069,017 A
  • Filed: 01/14/1977
  • Issued: 01/17/1978
  • Est. Priority Date: 01/14/1977
  • Status: Expired due to Term
First Claim
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1. A colorimetric method for the detection of bilirubin in an aqueous liquid which comprisesa. contacting in a reagent zone said aqueous liquid and an interactive mordant composition for bilirubin, such interactive mordant composition having one or more binding sites for bilirubin and comprising a hydrophobic organic matrix and at least one charge-bearing cationic group,whereby bilirubin is mordanted, said mordanted bilirubin exhibiting an absorption peak shifted at least about 10 nm. from that of free bilirubin and a molar extinction coefficient at least about 50 percent higher than that of free bilirubin, andb. colorimetrically detecting said mordanted bilirubin.

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