Device for controlling the particle flow in an apparatus for measuring the properties of particles suspended in liquid

• US 4,070,617 A
• Filed: 08/03/1976
• Issued: 01/24/1978
• Est. Priority Date: 05/08/1974
• Status: Expired due to Term

• Current Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Original Assignee: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften e.V. (Max-Planck-Gesellschaft zur Frderung der Wissenschaften e)
• Inventors: Glossner, Ewald, Kachel, Volker
• Primary Examiner(s): Strecker, Gerard R.
• Application Number:
  US05/711,224
• Time in Patent Office: 539 Days
• Field of Search: 324/71 CP, 356/102, 235/92 PC, 73/432 PS, 128/2 G
• US Class Current: 324/71.1
• CPC Class Codes: G01N 15/12 by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
• CPC Class Codes: G01N 15/131 Details G01N15/13 takes precedence

Abstract

An apparatus for investigating the properties of particles held in liquid spension using the Coulter process, U.S. Pat. No. 2,656,508, wherein the flowrate of the particle suspension into the particle-free electrolyte carrier depends on the pressure difference between these two liquids. This pressure difference is made readily adjustable by setting the height of the level of the electrolyte by means of a separate, vertically movable chamber attached with a flexible lead to the main measuring chamber. The main measuring chamber is closed to the atmosphere, permitting maintenance of a pressure differential with respect to the atmosphere and with respect to the particle suspension.

Claims

1. In an apparatus for the measurement of properties of particles held in suspension including:

a housing defining therein a main measuring chamber separated into a first and a second chamber;

a separating wall separating both chambers and having a measuring aperture formed therein connecting the first and second chamber, wherein the first chamber is supplied with particle-free electrolyte;

means connected to the second chamber for maintaining the pressure in the second chamber below the pressure in the first chamber causing thereby the electrolyte to flow from the first chamber through the measuring aperture into the second chamber;

a first electrode mounted with the first chamber;

a second electrode mounted with the second chamber; and

a supply capillary tube subject to the surrounding atmospheric pressure for receiving the suspension and having an outlet, said supply capillary tube being mounted to extend with its outlet into the first chamber such that to allow the suspension to enter into the first chamber and be carried through the measuring aperture with the electrolyte, when the pressure in the supply capillary tube exceeds the pressure in the first chamber around the outlet, the improvement comprising;

a. said housing constructed to pressure isolate the first chamber from the surrounding atmosphere; and

b. a source of variable pressure connected to the first chamber for providing within said first chamber a variable pressure less than atmospheric pressure and above the pressure in the second chamber to effect the flow of particles containing suspension from the supply capillary tube into the first measuring chamber.

2. An improved apparatus as defined in claim 1, wherein the separating wall has substantially the shape of a cone whose apex points in the direction of said second chamber, said cone being further provided with an outlet aperture near the base of the cone;

whereby said outlet aperture lies substantially higher than the measuring aperture when the apparatus is in its normal operating position.

3. An improved apparatus as defined in claim 1, wherein the improvement further comprises:

g. flushing means mounted to the housing and extending into the second chamber;

whereby fluid may be moved in and around the measuring aperture.

4. An improved apparatus as defined in claim 3, wherein said flushing means includes a compressible fluid container;

whereby fluid may be moved out of said flushing means by compression of said compressible container.

5. An improved apparatus as defined in claim 1, wherein the improvement further comprises:

h. a capillary flushing tube mounted to the housing and extending into the first chamber;

whereby fluid may be moved in and around said measuring aperture.

6. An improved apparatus as defined in claim 5, wherein the improvement further comprises:

i. a source of reduced pressure;

j. control means disposed adjacent to said main measuring chamber and capable of two control positions;

a first control position, in which communication is established between the antechamber and the first chamber and also between the exterior of the apparatus and the second chamber;

and a second control position, in which communication between the antechamber and the first chamber is interrupted and a communication is established between the antechamber and said capillary flushing tube, and between said source of reduced pressure and the second chamber, whereas the communication of the second chamber with the exterior of the apparatus is interrupted.

7. An improved apparatus as defined in claim 1, wherein the improvement further comprises:

k. a clamping and sealing element, mounted in the wall of the housing at the first chamber;

whereby said supply capillary tube is inserted in a bore within said clamping and sealing element so as to extend into the first chamber.

8. An improved apparatus as defined in claim 1, wherein the improvement further comprises:

l. a supply container, for holding a supply of particle suspension, said supply container being provided with an inlet stud and an insertion stud, respectively at opposite ends thereof, and of circular cross-section.

9. An improved apparatus as defined in claim 8, wherein the cross-section of said inlet stud and of said insertion stud is sufficiently small that the particle suspension contained in said supply container is retained therein unless it is placed in contact with fluid external thereto.

10. An improved apparatus as defined in claim 8, wherein the improvement further comprises:

m. a bushing, mounted in the wall of the housing at the first chamber; and

n. a second clamping and sealing element disposed coaxially in the bore of said bushing, whereby said container for particle suspension may be inserted into said second clamping and sealing element, thereby permitting flow of particle suspension from said container to said supply capillary tube.

11. An improved apparatus as defined in claim 9, wherein the improvement further comprises:

o. photoelectric means, including a light source and a photosensitive cell, disposed at said inlet stud and at said insertion stud in such a manner that a signal is generated by said photoelectric means to indicate the presence of particle suspension in the respective stud.

12. An improved apparatus as defined in claim 11, wherein the signals generated by the photosensitive cells serve to initiate and terminate the analysis of the signals generated by the first and second electrodes.

13. An improved apparatus as defined in claim 1, wherein the improvement further comprises:

p. a removable insert formed as one portion of the housing, said insert defining a bore and having a lower wall which forms the separating wall containing the measuring aperture, said insert forming with the remainder of the housing, said first and second chambers;

q. an upper member, centrally inserted into said removable insert and provided with a central bore, the lower part of which is capable of holding said supply capillary tube; and

r. a clamping and sealing member, removably inserted into said upper member, provided with a central bore and also provided with a surface which limits the depth of insertion of a container of particle suspension into said central bore.

14. An improved apparatus as defined in claim 13, wherein said first electrode is mounted on an interior surface of said upper member.

15. An improved apparatus as defined in claim 14, wherein said first electrode is disposed in the central bore within said upper member, and has the form of an annulus concentric with said supply capillary tube.

16. An improved apparatus as defined in claim 15, wherein the improvement further comprises:

s. a locking ring, disposed to cooperate with said removable insert in the manner of a bayonet closure so as to clamp said upper member to said removable insert.

17. An improved apparatus as defined in claim 16, wherein the improvement further comprises:

t. a flushing capillary tube, wherein said upper member contains a first internal channel for supply or removal of electrolyte from the first chamber and a second internal channel for supplying flushing fluid to said flushing capillary tube.

18. An improved apparatus as defined in claim 17, wherein the improvement further comprises:

u. a supply container, for holding particle suspension, removably and sealingly disposed in said central bore of said removable insert.

19. An improved apparatus as defined in claim 1, wherein the means for providing a pressure less than atmospheric pressure within the first chamber comprises:

c. an antechamber, which is filled with particle-free electrolyte, said particle-free electrolyte being subject to atmospheric pressure;

d. control means for keeping the level of electrolyte within the antechamber at a position, predetermined and constant with respect to the antechamber, irrespective of the height of the antechamber with respect to the first chamber;

e. connecting means for supplying electrolyte from the antechamber to the first chamber; and

f. means for varying the height of the antechamber with respect to the first chamber.

20. An improved apparatus as defined in claim 19, wherein said control means are within said antechamber, and include:

i. valve means; and

ii. float means,

whereby said float means closes said valve means when the level of the electrolyte has reached a predetermined value, thereby stopping the flow of electrolyte to the first chamber.

21. An improved apparatus as defined in claim 19, wherein the improvement further comprises:

f. a drip chamber, disposed in said antechamber, for providing electrical un-coupling of the electrolyte in said antechamber from its source of supply.

22. An improved apparatus as defined in claim 19, wherein said antechamber is provided with a vent opening, creating a communication to the exterior thereof.