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Membrane for enzyme electrodes

  • US 4,073,713 A
  • Filed: 07/26/1976
  • Issued: 02/14/1978
  • Est. Priority Date: 09/24/1975
  • Status: Expired due to Term
First Claim
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1. A polarographic cell structure for use in polarographic analysis of an unknown comprising an electrically insulating support means, and electrode means mounted in said support means, said electrode means mounted in said support means, said electrode means including means defining an active exposed working face, wherein the improvement comprises:

  • a laminated membrane of a total thickness of less than 25 microns positioned between said working face and said unknown, said membrane consisting essentially of a first layer of an essentially homogeneous material selected from the group consisting of silicone rubber, methyl methacrylate, and cellulose acetate and having a thickness of less than 2 microns, a second layer of a support material which permits passage of low molecular weight substances but excludes high molecular weight substances, said support material having a thickness of between 1 and 20 microns, and an adhesive layer positioned between and bonding said first and second layers, an enzyme being included in said adhesive layer.

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