Method, composition, and device for determining the specific gravity of a liquid
First Claim
1. A method for determining the specific gravity of a urine sample, which method comprises the steps of:
- (a) contacting said sample with a predetermined concentration of an enzyme and of a reagent upon which said enzyme exerts its catalytic activity, the rate of reaction of said reagent in the presence of said enzyme being affected by the aggregate solute content of said sample,(b) determining the rate of said reaction in contact with said sample, and(c) comparing such determined reaction rate to the rates of said reaction when carried out in contact respectively with samples of urine having known different specific gravities.
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Abstract
A method, composition, and device for determining the specific gravity of a liquid. In the method, a sample of the liquid to be tested is contacted with standardized quantities of substances which participate in a reaction that is affected by the level of solute in said liquid. The resulting effect of the solute on the reaction is a reproducible function of the concentration or amount of solute in the sample and relates to the specific gravity of the liquid tested. A unitized test composition is provided comprising at least one substance which participates in the standardized reaction. The standardized reaction is preferably chemical in nature and, in such a case, the test composition preferably includes an indicator responsive to a product of the chemical reaction.
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Citations
31 Claims
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1. A method for determining the specific gravity of a urine sample, which method comprises the steps of:
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(a) contacting said sample with a predetermined concentration of an enzyme and of a reagent upon which said enzyme exerts its catalytic activity, the rate of reaction of said reagent in the presence of said enzyme being affected by the aggregate solute content of said sample, (b) determining the rate of said reaction in contact with said sample, and (c) comparing such determined reaction rate to the rates of said reaction when carried out in contact respectively with samples of urine having known different specific gravities. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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- 11. A test composition for determining the specific gravity of a urine sample, which composition comprises (1) a predetermined quantity of an enzyme and of a reagent upon which said enzyme exerts its catalytic effect, the rate of reaction of said reagent in the presence of said enzyme being affected by the aggregate solute content of said sample, and (2) indicator means responsive to the presence of a product of said reaction.
Specification