Automatic cell analyzer method

• US 4,085,006 A
• Filed: 11/06/1974
• Issued: 04/18/1978
• Est. Priority Date: 11/06/1974
• Status: Expired due to Term

• Current Assignee: Isomedics Incorporated
• Original Assignee: Isomedics Incorporated
• Inventors: Inbar, Dan, Bruck, Avraham, Mindick, Leo R., Koss, Leopold G.
• Primary Examiner(s): Tanenholtz, Alvin E.
• Application Number:
  US05/521,518
• Time in Patent Office: 1,259 Days
• Field of Search: 195/103.7, 195/127
• US Class Current: 435/35
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/574 for cancer

Abstract

A cell analyzer which automatically processes and evaluates cell samples for possible indications of malignancy and other abnormalities is disclosed. Cell samples are prepared in a suspension and are tagged with an appropriate radioactive stain or appropriate biochemical. Counting, filtering and drying steps under computer control are followed by the taking of a radioactive count with respect to a normalized number of cells. The results of the radioactive counting determine whether the particular sample is classified as "positive" or "negative".

Claims

1. A method for analyzing cells for determination of cell character, comprising the steps of selecting a sample of cells, placing the cells in a fixative solution to form a suspension, ultrasonicating the cells under conditions to achieve dispersion of cells without disrupting cell membrane integrity, incorporating in the suspension a radioactive biochemical which attaches to at least a portion of the cells, counting a pre-determined number of cells withdrawn from the radioactive suspension, depositing said counted cells at a draining location, transporting said counted and drained cells to a drying location and to a radioactive detection location, performing a radioactive count on said cells at said radioactive detection location and comparing said radioactive count in normalized form with a pre-determined setting to evaluate said sample to determine the character of said cells.

2. The method defined by claim 1 wherein said cell counting, depositing, transporting, draining, drying, radioactive detecting and comparing steps are performed under computer control, and said radioactive biochemical is acridine orange.

3. The method defined by claim 1 wherein said counting step includes analyzing said cells for size and including in the cell count only cells within a pre-determined size range.