Porous cellulose beads
First Claim
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1. A process for the preparation of porous cellulose beads suitable for use as a carrier of enzymes and other biological agents which comprises the steps of:
- a. dissolving a hydrolyzable cellulose derivative in an inert organic, water-miscible solvent to form a solution having a density greater than that of a precipitation solution the cellulose derivative to solvent ratio ranging from 1;
20 to 1;
3 weight/volume;
b. distributing said solution in the form of droplets into a precipitation solution whereby said cellulose derivative is precipitated in the form of uniformly porous beads;
c. separating the precipitated beads from said solution;
d. washing the separated porous beads with water;
e. hydrolyzing the washed beads to convert the beads to cellulose and to increase the active sites for attachment of enzymes and other biological agents;
f. washing the hydrolyzed beads to obtain porous cellulose beads having a uniformly distributed void space greater than 50% by volume.
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Abstract
Porous cellulose beads are prepared by distributing droplets of a solvent mixture containing a cellulose derivative into a precipitating solution to form porous beads which are then washed and hydrolyzed to form porous cellulose beads. The porous cellulose beads, which may be cross-linked, if desired, by suitable treatment, are useful carriers to which enzymes can be immobilized. The beads may also be used for the separation of enzymes, proteins, nucleic acids and the like, or to remove metal ions from dilute mining solutions.
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20 Claims
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1. A process for the preparation of porous cellulose beads suitable for use as a carrier of enzymes and other biological agents which comprises the steps of:
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a. dissolving a hydrolyzable cellulose derivative in an inert organic, water-miscible solvent to form a solution having a density greater than that of a precipitation solution the cellulose derivative to solvent ratio ranging from 1;
20 to 1;
3 weight/volume;b. distributing said solution in the form of droplets into a precipitation solution whereby said cellulose derivative is precipitated in the form of uniformly porous beads; c. separating the precipitated beads from said solution; d. washing the separated porous beads with water; e. hydrolyzing the washed beads to convert the beads to cellulose and to increase the active sites for attachment of enzymes and other biological agents; f. washing the hydrolyzed beads to obtain porous cellulose beads having a uniformly distributed void space greater than 50% by volume. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification